College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2023 Dec 14;24(24):17463. doi: 10.3390/ijms242417463.
The PEBP gene family plays a significant role in regulating flower development and formation. To understand its function in and flowering, we identified 22 PEBP genes (11 s and 11 s) from both species. We conducted analyses on their conserved domains and motifs, phylogenetic relationships, chromosome distribution, collinear correlation, and elements. The classification results showed that the 22 s were mainly divided into three clades, as follows: FT, MFT, and TFL1. A sequence analysis showed that most PEBP proteins contained five conserved domains, while a gene structure analysis revealed that 77% of the total PEBP genes contained four exons and three introns. The promoter regions of the 22 s contained several elements related to hormone induction and light response. This suggests these s could play a role in regulating flower development by controlling photoperiod and hormone levels. Additionally, a collinearity analysis revealed three pairs of duplicate genes in the genomes of both and . Furthermore, RT-qPCR has found to influence the regulatory effect of s on the development of flower organs (sepals, petals, lip, ovary, and gynostemium) during the flowering process (bud, transparent stage, and initial bloom). The results obtained imply that and play a role in the initial bloom and that may inhibit flowering processes. Moreover, may potentially be involved in the development of reproductive functionality. s have regulatory functions that modulate flowering. FT initiates plant flowering by mediating photoperiod and temperature signals, while TFL1 inhibits flowering processes. These findings provide clues for future studies on flower development in
PEBP 基因家族在调控花发育和形成中起着重要作用。为了了解其在 和 开花中的功能，我们从两个物种中鉴定出 22 个 PEBP 基因（11s 和 11s）。我们对它们的保守结构域和基序、系统发育关系、染色体分布、共线性关联和 元件进行了分析。分类结果表明，这 22 个基因主要分为三个分支，分别是：FT、MFT 和 TFL1。序列分析表明，大多数 PEBP 蛋白包含五个保守结构域，而基因结构分析表明，总共有 77%的 PEBP 基因包含四个外显子和三个内含子。22 个基因的启动子区域包含了几个与激素诱导和光响应相关的 元件。这表明这些基因可能通过控制光周期和激素水平在花发育的调控中发挥作用。此外，共线性分析揭示了 和 基因组中存在三对重复基因。此外，RT-qPCR 发现这些基因在开花过程中（芽、透明期和初花期）对花器官（萼片、花瓣、唇瓣、子房和雌蕊）的发育有调节作用。研究结果表明，和 在初花期发挥作用，而可能抑制开花过程。此外，可能潜在地参与生殖功能的发育。这些基因具有调节开花的功能。FT 通过介导光周期和温度信号启动植物开花，而 TFL1 则抑制开花过程。这些发现为未来研究 花发育提供了线索。
