Rodrigues Maria João, Castañeda-Loaiza Viana, Fernandes Eliana, Custódio Luísa
Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Campus of Gambelas, 8005-139 Faro, Portugal.
Plants (Basel). 2023 Jun 19;12(12):2366. doi: 10.3390/plants12122366.
L. (golden samphire) is an edible aromatic halophyte species with confirmed nutritional and medicinal properties attributed to the presence of important metabolites, including proteins, carotenoids, vitamins, and minerals. Therefore, this study aimed at establishing a micropropagation protocol for golden samphire that can serve as a nursery approach to its standardized commercial cultivation. For that purpose, a complete regeneration protocol was developed by improving shoot multiplication from nodal explants, rooting, and acclimatization methodologies. The treatment with BAP alone induced the maximum shoot formation (7-7.8 shoots/explant), while IAA treatment increased the shoot height (9.26-9.5 cm). Furthermore, the treatment that coupled best shoot multiplication (7.8 shoots/explant) and highest shoot height (7.58 cm) was MS medium supplemented with 0.25 mg/L BAP. Moreover, all shoots produced roots (100% rooting), and multiplication treatments did not exert significant effect on root length (7.8-9.7 cm/plantlet). Moreover, by the end of the rooting phase, plantlets cultivated with 0.25 mg/L BAP had the highest shoot number (4.2 shoots/plantlet), and plantlets from 0.6 mg/L IAA + 1 mg/L BAP presented the highest shoot height (14.2 cm) similar to control plantlets (14.0 cm). The survival up to the ex-vitro acclimatization stage was increased from 9.8% (control) to 83.3%, when plants were treated with a paraffin solution. Nevertheless, the in vitro multiplication of golden samphire is a promising way for its rapid propagation and can be used as a nursery method, contributing to the development of this species as an alternative food and medicinal crop.
滨海刺芹是一种可食用的芳香盐生植物，因其含有蛋白质、类胡萝卜素、维生素和矿物质等重要代谢产物而具有确切的营养和药用特性。因此，本研究旨在建立滨海刺芹的微繁殖方案，作为其标准化商业种植的育苗方法。为此，通过改进节段外植体的芽增殖、生根和驯化方法，制定了完整的再生方案。单独使用苄氨基嘌呤（BAP）处理诱导出最多的芽形成（7 - 7.8个芽/外植体），而吲哚乙酸（IAA）处理增加了芽的高度（9.26 - 9.5厘米）。此外，结合最佳芽增殖（7.8个芽/外植体）和最高芽高度（7.58厘米）的处理是添加0.25毫克/升BAP的MS培养基。此外，所有芽都生根（100%生根），增殖处理对根长（7.8 - 9.7厘米/小植株）没有显著影响。此外，在生根阶段结束时，用0.25毫克/升BAP培养的小植株芽数最多（每个小植株4.2个芽），用0.6毫克/升IAA + 1毫克/升BAP培养的小植株芽高度最高（14.2厘米），与对照小植株（14.0厘米）相似。当用石蜡溶液处理植物时，离体驯化阶段的存活率从9.8%（对照）提高到了83.3%。然而，滨海刺芹的离体繁殖是其快速繁殖的一种有前途的方法，可作为育苗方法，有助于将该物种发展成为替代粮食和药用作物。
