Instituto de Histología y Embriología de Mendoza (IHEM), Universidad Nacional de Cuyo, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza 5500, Argentina.
Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza 5500, Argentina.
Viruses. 2023 May 31;15(6):1295. doi: 10.3390/v15061295.
Gumboro illness is caused by the highly contagious immunosuppressive infectious bursal disease virus (IBDV), which affects the poultry industry globally. We have previously shown that IBDV hijacks the endocytic pathway to construct viral replication complexes on endosomes linked to the Golgi complex (GC). Then, analyzing crucial proteins involved in the secretory pathway, we showed the essential requirement of Rab1b, the Rab1b downstream effector Golgi-specific BFA resistance factor 1 (GBF1), and its substrate, the small GTPase ADP-ribosylation factor 1 (ARF1), for IBDV replication. In the current work, we focused on elucidating the IBDV assembly sites. We show that viral assembly occurs within single-membrane compartments closely associated with endoplasmic reticulum (ER) membranes, though we failed to elucidate the exact nature of the virus-wrapping membranes. Additionally, we show that IBDV infection promotes the stress of the ER, characterized by an accumulation of the chaperone binding protein (BiP) and lipid droplets (LDs) in the host cells. Overall, our results represent further original data showing the interplay between IBDV and the secretory pathway, making a substantial contribution to the field of birnaviruses-host cell interactions.
传染性法氏囊病病毒（IBDV）是一种高度传染性的免疫抑制性传染性bursal 病病毒，它会影响全球的家禽养殖业。我们之前已经表明，IBDV 劫持了内吞作用途径，在与高尔基体复合体（GC）相连的内体上构建病毒复制复合物。然后，通过分析参与分泌途径的关键蛋白，我们表明 Rab1b、Rab1b 的下游效应物高尔基体特异性 BFA 抗性因子 1（GBF1）及其底物、小分子 GTP 酶 ADP-核糖基化因子 1（ARF1）对于 IBDV 复制是必不可少的。在当前的工作中，我们专注于阐明 IBDV 的组装部位。我们表明，病毒组装发生在与内质网（ER）膜紧密相关的单膜隔室内，但我们未能阐明病毒包裹膜的确切性质。此外，我们表明 IBDV 感染会导致 ER 应激，表现为伴侣结合蛋白（BiP）和脂滴（LD）在宿主细胞中的积累。总的来说，我们的研究结果提供了进一步的原始数据，展示了 IBDV 与分泌途径之间的相互作用，为双 RNA 病毒-宿主细胞相互作用领域做出了重要贡献。
