Associate Professor, Department of Histology with Cytology and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
Associate Professor, Head of the Department of Histology with Cytology and Embryology; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
Sovrem Tekhnologii Med. 2023;15(1):30-36. doi: 10.17691/stm2023.15.1.04. Epub 2023 Jan 28.
is to study structural alterations of autologous omental adipose tissue in a silicon conduit and to evaluate its possible use for regeneration of the sciatic nerve in diastasis.
Mature outbred male Wistar rats have been used in the study. The animals were divided into 7 experimental groups with complete transection of the sciatic nerve on the right side at the mid-third level of the thigh. The ends of the transected nerve were pulled apart, inserted into a silicon conduit, and secured to the epineurium. The conduit of group 1 (control) was filled with a saline solution; in group 2, it was filled with an autologous omental adipose tissue with saline solution. Intravital labeling of the omental adipose tissue with the lipophilic PKH 26 dye (in group 3) was used for the first time to find out whether the omental cells were involved in formation of the regenerating nerve. Diastasis in groups 1-3 was 5 mm, the postoperative period was 14 weeks. The dynamics of the omental adipose tissue changes in groups 4-7 was assessed by placing the omental tissues into the conduit covering 2 mm of diastasis. The postoperative period was 4, 14, 21, and 42 weeks.
In group 2 (omental adipose tissue + saline), the clinical condition of the damaged limb after 14 weeks may be evaluated as satisfactory and approximating to the intact parameters as compared to group 1 where the conduit was filled with a saline solution only. The sum of large and medium-sized nerve fibers in group 2 was 2.7 times greater than that in group 2. The milled omental adipose tissue inside the conduit changed its volume and structure in nerve diastasis and was constantly utilized up to complete elimination over time. The omental cells integrated into the newly formed nerve in the graft area.
As a graft, the adipose tissue of the autologous omentum produces a stimulating effect on the post-traumatic regeneration of the sciatic nerve.
研究自体大网膜脂肪组织在硅管中的结构改变，并评估其在坐骨神经分离再生中的可能用途。
本研究使用成熟的近交系雄性 Wistar 大鼠。动物分为 7 个实验组，右侧大腿中三分之一处完全横断坐骨神经。将切断的神经末端拉开，插入硅管，并固定在神经外膜上。第 1 组（对照）的导管充满生理盐水；第 2 组导管中填充含有生理盐水的自体大网膜脂肪组织。首次使用亲脂性 PKH26 染料对大网膜脂肪组织进行活体标记，以确定大网膜细胞是否参与再生神经的形成。第 1 组至第 3 组的分离距离为 5mm，术后 14 周。第 4 组至第 7 组通过将大网膜组织放入覆盖 2mm 分离距离的导管中来评估大网膜组织的动态变化。术后 4、14、21 和 42 周。
第 2 组（大网膜脂肪组织+生理盐水），与仅用生理盐水填充导管的第 1 组相比，14 周后受损肢体的临床状况可能评估为满意，接近完整参数。第 2 组中较大和中等大小神经纤维的总和是第 2 组的 2.7 倍。在神经分离过程中，导管内研磨的大网膜脂肪组织改变了其体积和结构，随着时间的推移，其不断被利用直至完全消除。大网膜细胞整合到移植物区域新形成的神经中。
作为移植物，自体大网膜脂肪组织对坐骨神经创伤后再生具有刺激作用。
