分化脂肪来源干细胞促进周围神经再生。
Differentiated adipose-derived stem cells promote peripheral nerve regeneration.
机构信息
Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa, Japan.
出版信息
Muscle Nerve. 2020 Jul;62(1):119-127. doi: 10.1002/mus.26879. Epub 2020 Apr 18.
INTRODUCTION
Many reports have indicated that adipose-derived stem cells (ADSCs) are effective for nerve regeneration. We investigated nerve regeneration by combining a polyglycolic acid collagen (PGA-c) tube, which is approved for clinical use, and Schwann cell-like differentiated ADSCs (dADSCs).
METHODS
Fifteen-millimeter-long gaps in the sciatic nerve of rats were bridged in each group using tubes (group I), with tubes injected with dADSCs (group II), or by resected nerve (group III).
RESULTS
Axonal outgrowth was greater in group II than in group I. Tibialis anterior muscle weight revealed recovery only in group III. Latency in nerve conduction studies was equivalent in group II and III, but action potential was lower in group II. Transplanted dADSCs maintained Schwann cell marker expression. ATF3 expression level in the dorsal root ganglia was equivalent in groups II and III.
DISCUSSION
dADSCs maintained their differentiated state in the tubes and are believed to have contributed to nerve regeneration.
简介
许多报告表明脂肪来源干细胞（ADSCs）可有效促进神经再生。我们通过联合使用聚乙醇酸胶原（PGA-c）管（已批准用于临床）和 Schwann 细胞样分化的 ADSCs（dADSCs）来研究神经再生。
方法
在每组大鼠的坐骨神经中桥接 15 毫米长的间隙，使用管（第 I 组），用 dADSCs 注射管（第 II 组）或切除神经（第 III 组）。
结果
第 II 组的轴突生长大于第 I 组。在前胫骨肌肉重量仅在第 III 组中恢复。神经传导研究中的潜伏期在第 II 组和第 III 组中相等，但动作电位在第 II 组中较低。移植的 dADSCs 保持 Schwann 细胞标志物的表达。第 II 组和第 III 组背根神经节中的 ATF3 表达水平相当。
讨论
dADSCs 在管中保持其分化状态，据信对神经再生有贡献。
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