Khalaf Samar S, Shalaby Ola A, Hassan Ahmed R, El-Kherbetawy Mohamed K, Mehanna Eman T
Department of Biochemistry, Faculty of Pharmacy, Heliopolis University, Cairo, Egypt.
Department of Medicinal and Aromatic Plants, Desert Research Center, El-Matariya, 11753, Cairo, Egypt.
J Tradit Complement Med. 2023 Mar 8;13(4):397-407. doi: 10.1016/j.jtcme.2023.03.005. eCollection 2023 Jul.
() is an imperative plant with many medicinal uses. The current study aimed to investigate the protective effects of the stem bark of and its fractions in a high fat diet (HFD) rat model.
Seventy-two male albino rats were randomly divided into 9 groups, 8 rats per each. Group 1 was the normal control and received standard balanced diet. All the remaining groups were fed HFD for 8 weeks to induce obesity. Group 2 served as the HFD control group, group 3 received orlistat (5 mg/kg/day), groups 4 and 5 received total extract of stem bark (250 and 500 mg/kg). Groups 6 and 7 received ethyl acetate fraction (250 and 500 mg/kg), while groups 8 and 9 received butanol fraction (250 and 500 mg/kg).
Both doses of the ethyl acetate fraction of the stem bark of significantly decreased the body weight, blood glucose, lipid profile and improved insulin sensitivity. Levels of MDA, leptin and inflammatory cytokines were significantly decreased by the ethyl acetate fraction while adiponectin and HDL-C were significantly increased relative to the HFD control group. Both doses of the ethyl acetate fraction significantly abolished HDF induced oxidative stress and normalized the values of antioxidant enzymes. Furthermore, metabolic profiling of the ethyl acetate fraction was performed by UHPLC/Q-TOF-MS. In conclusion, the ethyl acetate fraction of stem bark possessed antioxidant, anti-inflammatory and insulin sensitizing properties in HFD rat model.
（某植物名）是一种具有多种药用价值的重要植物。本研究旨在探讨（某植物名）茎皮及其提取物在高脂饮食（HFD）大鼠模型中的保护作用。
72只雄性白化大鼠随机分为9组，每组8只。第1组为正常对照组，给予标准平衡饮食。其余所有组均给予高脂饮食8周以诱导肥胖。第2组作为高脂饮食对照组，第3组给予奥利司他（5毫克/千克/天），第4组和第5组给予（某植物名）茎皮总提取物（250和500毫克/千克）。第6组和第7组给予乙酸乙酯提取物（250和500毫克/千克），而第8组和第9组给予正丁醇提取物（250和500毫克/千克）。
（某植物名）茎皮的乙酸乙酯提取物的两种剂量均显著降低了体重、血糖、血脂水平，并改善了胰岛素敏感性。相对于高脂饮食对照组，乙酸乙酯提取物显著降低了丙二醛、瘦素和炎症细胞因子的水平，同时显著提高了脂联素和高密度脂蛋白胆固醇的水平。乙酸乙酯提取物的两种剂量均显著消除了高脂饮食诱导的氧化应激，并使抗氧化酶的值恢复正常。此外，通过超高效液相色谱/四极杆飞行时间质谱对乙酸乙酯提取物进行了代谢谱分析。总之，（某植物名）茎皮的乙酸乙酯提取物在高脂饮食大鼠模型中具有抗氧化、抗炎和胰岛素增敏特性。
