克罗恩病患者与健康个体肠道病毒群落的比较。

Comparison of the gut virus communities between patients with Crohn's disease and healthy individuals.

作者信息

Ding Yuzhu, Wan Mengtian, Li Zheng, Ma Xiao, Zhang Wen, Xu Min

机构信息

Department of Gastroenterology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, Jiangsu, China.

Qinghai Institute for Endemic Disease Prevention and Control, Xining, Qinghai, China.

出版信息

Front Microbiol. 2023 Jun 15;14:1190172. doi: 10.3389/fmicb.2023.1190172. eCollection 2023.

DOI:10.3389/fmicb.2023.1190172

PMID:37396350

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10311494/

Abstract

INTRODUCTION

The escalating incidence of Crohn's disease (CD), a debilitating ailment that ravages individuals and their families, has become a formidable issue over recent decades.

METHOD

In this study, fecal samples from patients with CD and healthy individuals were investigated by means of viral metagenomics.

RESULTS

The fecal virome was analyzed and some suspected disease-causing viruses were described. A polyomavirus named HuPyV with 5,120 base pairs (bp) was found in the disease group. In a preliminary analysis employing large T region-specific primers, it was found that HuPyV was present in 3.2% (1/31) of healthy samples and 43.2% (16/37) of disease samples. Additionally, two other viruses from the anellovirus and CRESS-DNA virus families were found in fecal samples from CD patients. The complete genome sequences of these two viruses were described respectively, and the phylogenetic trees have been built using the anticipated amino acid sequences of the viral proteins.

DISCUSSION

Further research is required to elucidate the relationship between these viruses and the onset and development of Crohn's disease.

摘要

引言

克罗恩病（CD）的发病率不断攀升，这是一种折磨患者及其家庭的使人衰弱的疾病，在最近几十年已成为一个严峻的问题。

方法

在本研究中，通过病毒宏基因组学对克罗恩病患者和健康个体的粪便样本进行了调查。

结果

对粪便病毒组进行了分析，并描述了一些疑似致病病毒。在疾病组中发现了一种名为HuPyV的多瘤病毒，其长度为5120个碱基对（bp）。在使用大T区域特异性引物的初步分析中，发现HuPyV存在于3.2%（1/31）的健康样本和43.2%（16/37）的疾病样本中。此外，在克罗恩病患者的粪便样本中还发现了另外两种来自环曲病毒科和CRESS-DNA病毒科的病毒。分别描述了这两种病毒的完整基因组序列，并使用病毒蛋白的预测氨基酸序列构建了系统发育树。