Bolanle Ajayi Olubunmi, Grace Oluwafemi Adekemi
Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria.
Department of Biochemistry, Landmark University, Omu-Aran, Nigeria.
Nutr Health. 2025 Jun;31(2):429-438. doi: 10.1177/02601060231185607. Epub 2023 Jul 4.
BackgroundTurmeric ( has been recognized as a plant with high medicinal value and it has been used in the prevention and treatment of many diseases. Different studies have shown that turmeric has both therapeutic and preventive effects on peptic ulcer. However, there are controversial reports about the anti-ulcerogenic potential of turmeric. Some studies also suggested that turmeric could be ulcerogenic when consumed in large quantity with no mention of the concentration at which this could take place.AimThis study examined the influences of different concentration of turmeric rhizome powder supplemented diet on the gene expressions of both anti-ulcer and ulcer biomarkers in indomethacin-induced ulcerated rats.MethodsThe study was conducted through prophylactic treatment of test groups with turmeric at different percentage (1%, 2%, 5%, and 10%) for 28 days. Thirty-five rats were randomly divided into seven groups namely A, B, C, D (1%, 2%, 5%, and 10% groups respectively), E (standard drug group (STD)), F (ulcerogenic group) and G (normal control group (CTL)). At the end of 28 days, rats were fasted overnight and ulcer was induced in all the groups except group G by oral administration of 60mg/kg b.w (body weight) of indomethacin. The expression of defensive (Cyclo-oxygenase-1, MUCIN, and Hyme-oxygenase-1) and destructive factors (Pepsin) were then analyzed.ResultsResults showed that consumption of TRPSD at 1-5% increased the gene expression of protective factors when compared with animals in group F. Furthermore, Prophylactic treatment of test groups with TRPSD at 1%-5% significantly suppressed the gene expression of pepsin in comparison with animals in group F. However, TRPSD consumption at 10% down-regulated the gene expression of those protective factors. Similarly, at 10%, pepsin gene expression was not suppressed when compared with animals in group F. Conclusively, TRPSD could be gastro protective at 1%-5%. However, these potentials were abrogated in animals in group D indicating the ulcerogenic potential of turmeric at this concentration (10%) and its capability to enhance ulcerogenic action of indomethacin.ConclusionTurmeric rhizome powder (TRP) have anti-ulcerogenic potential and gastro-protective effect when consumed in appropriate concentration. Consumption of TRP at 10% concentration could enhance ulcerogenic action of indomethacin (NSAIDs) thus predisposing to ulcer. Effects of turmeric rhizome powder supplemented diet (TRPSD) on the mRNA expression of protective agents (cyclo-oxygenase-1 (COX-1), mucin, and inducible heme-oxygenase (HO-1)) and destructive factor (pepsin), in indomethacin-induced ulcerated Wistar rats were explored in this paper. These were determined by prophylactic treatment of test groups with turmeric at different levels (1%, 2%, 5%, and 10%) for 28 days. Thirty-five rats were randomly divided into seven groups namely A, B, C, D (1%, 2%, 5%, and 10% groups respectively), E (standard drug group (STD)), F (ulcerogenic group) and G (normal control group (CTL)). The rats were fasted overnight and ulcer was induced in all the groups except group G by oral administration of 60mg/kg b.w (body weight) of indomethacin. The expression of defensive (Cyclo-oxygenase-1, MUCIN, and Hyme-oxygenase-1) and destructive factors (Pepsin) were then analyzed. Results showed that consumption of TRPSD at 1%-5% increased the gene expression of protective factors when compared with animals in group F. Furthermore, Prophylactic treatment of test groups with TRPSD at 1%-5% significantly suppressed the gene expression of pepsin in comparison with animals in group F. However, TRPSD consumption at 10% down-regulated the gene expression of those protective factors. Similarly, at 10%, pepsin gene expression was not suppressed when compared with animals in group F. Conclusively, TRPSD could be gastro protective at 1%-5%. However, these potentials were abrogated in animals in group D indicating the ulcerogenic potential of turmeric at this concentration (10%) and its capability to enhance ulcerogenic action of indomethacin.
背景
姜黄已被公认为具有高药用价值的植物，并已用于预防和治疗多种疾病。不同的研究表明，姜黄对消化性溃疡具有治疗和预防作用。然而，关于姜黄的抗溃疡潜力存在有争议的报道。一些研究还表明，大量食用姜黄可能会导致溃疡，但未提及发生这种情况的浓度。
目的
本研究考察了不同浓度姜黄根茎粉补充饮食对消炎痛诱导的溃疡大鼠抗溃疡和溃疡生物标志物基因表达的影响。
方法
该研究通过对试验组进行不同百分比（1%、2%、5%和10%）的姜黄预防性治疗28天来进行。35只大鼠随机分为七组，即A、B、C、D组（分别为1%、2%、5%和10%组）、E组（标准药物组（STD））、F组（致溃疡组）和G组（正常对照组（CTL））。在28天结束时，大鼠禁食过夜，除G组外，所有组通过口服60mg/kg体重的消炎痛诱导溃疡。然后分析防御性（环氧化酶-1、粘蛋白和血红素加氧酶-1）和破坏性因子（胃蛋白酶）的表达。
结果
结果表明，与F组动物相比，食用1%-5%的姜黄根茎粉补充饮食可增加保护因子的基因表达。此外，与F组动物相比，用1%-5%的姜黄根茎粉补充饮食对试验组进行预防性治疗可显著抑制胃蛋白酶的基因表达。然而，食用10%的姜黄根茎粉会下调这些保护因子的基因表达。同样，在10%时，与F组动物相比，胃蛋白酶基因表达未受到抑制。总之，1%-5%的姜黄根茎粉补充饮食可能具有胃保护作用。然而这些潜力在D组动物中被消除，表明该浓度（10%）的姜黄具有致溃疡潜力及其增强消炎痛溃疡形成作用的能力。
结论
姜黄根茎粉在适当浓度下食用具有抗溃疡潜力和胃保护作用。食用10%浓度的姜黄根茎粉可增强消炎痛（非甾体抗炎药）的溃疡形成作用，从而易引发溃疡。本文探讨了姜黄根茎粉补充饮食（TRPSD）对消炎痛诱导的溃疡Wistar大鼠中保护剂（环氧化酶-1（COX-1）、粘蛋白和诱导型血红素加氧酶（HO-1））和破坏因子（胃蛋白酶）mRNA表达的影响。这些是通过对试验组进行不同水平（1%、2%、5%和10%）的姜黄预防性治疗28天来确定的。35只大鼠随机分为七组，即A、B、C、D组（分别为1%、2%、5%和10%组）、E组（标准药物组（STD））、F组（致溃疡组）和G组（正常对照组（CTL））。大鼠禁食过夜，除G组外，所有组通过口服60mg/kg体重的消炎痛诱导溃疡。然后分析防御性（环氧化酶-1、粘蛋白和血红素加氧酶-1）和破坏性因子（胃蛋白酶）的表达。结果表明，与F组动物相比，食用1%-5%的姜黄根茎粉补充饮食可增加保护因子的基因表达。此外，与F组动物相比，用1%-5%的姜黄根茎粉补充饮食对试验组进行预防性治疗可显著抑制胃蛋白酶的基因表达。然而，食用10%的姜黄根茎粉会下调这些保护因子的基因表达。同样，在10%时，与F组动物相比，胃蛋白酶基因表达未受到抑制。总之，1%-5%的姜黄根茎粉补充饮食可能具有胃保护作用。然而这些潜力在D组动物中被消除，表明该浓度（10%）的姜黄具有致溃疡潜力及其增强消炎痛溃疡形成作用的能力。
