Zhang Zichuan, Zhang Qian, Xue Yi, Fang Huiqing, Wu Zhongyin
Department of Stomatology, The 980th Hospital of PLA Joint Logistics Support Force (Bethune International Peace Hospital), Shijiazhuang, Hebei, PR China.
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Department of General Dentistry and Emergency, School of Stomatology, Air Force Medical University, Xi'an, PR China.
J Dent Sci. 2023 Jul;18(3):1103-1108. doi: 10.1016/j.jds.2022.11.016. Epub 2022 Nov 26.
BACKGROUND/PURPOSE: Recurrent aphthous stomatitis (RAS) is one of the most prevalent oral mucosa diseases with unknown etiology. Reduced glutathione (GSH) is a major intracellular non-protein physiological antioxidant, and it has been demonstrated that GSH deficiency may be related to cardiovascular, immune, and diabetes. The purpose of this investigation was to evaluate the potential roles of GSH, oxidized glutathione (GSSG), and glutathione reductase (GR) in the etiopathogenesis of minor recurrent aphthous stomatitis (MiRAS).
The study comprised 87 patients with idiopathic MiRAS and 90 race-, age-, and gender-matched healthy individuals. The spectrophotometric method was used to determine serum GSH and GSSG concentrations as well as GR activity. The GSSG/GSH ratios were subsequently computed. For statistical evaluation, the independent sample t test, Pearson's chi-square test, Mann-Whitney U test, Kruskal-Wallis H test, and Binary logistic regression analysis were used.
The serum GSSG level, GR activity and GSSG/GSH ratio were statistically higher in MiRAS patients, whereas the concentration of serum GSH was significantly decreased. With the exception of GR, serum GSSG, GSH, and GSSG/GSH were all significantly associated with MiRAS. Serum GSSG can be regarded as a risk factor, whereas serum GSH and GSSG/GSH maybe considered as protective factors against the occurrence of MiRAS.
GSSG may be a potential danger factor to MiRAS and GSH may be a protective factor, while GR may not play an important role in the aetiopathogenesis of MiRAS.
背景/目的：复发性阿弗他口炎（RAS）是最常见的口腔黏膜疾病之一，病因不明。还原型谷胱甘肽（GSH）是细胞内主要的非蛋白质生理性抗氧化剂，已有研究表明GSH缺乏可能与心血管、免疫及糖尿病相关。本研究旨在评估GSH、氧化型谷胱甘肽（GSSG）及谷胱甘肽还原酶（GR）在轻型复发性阿弗他口炎（MiRAS）发病机制中的潜在作用。
本研究纳入87例特发性MiRAS患者及90例种族、年龄和性别匹配的健康个体。采用分光光度法测定血清GSH和GSSG浓度以及GR活性，随后计算GSSG/GSH比值。采用独立样本t检验、Pearson卡方检验、Mann-Whitney U检验、Kruskal-Wallis H检验及二元逻辑回归分析进行统计学评估。
MiRAS患者血清GSSG水平、GR活性及GSSG/GSH比值在统计学上更高，而血清GSH浓度显著降低。除GR外，血清GSSG、GSH及GSSG/GSH均与MiRAS显著相关。血清GSSG可被视为危险因素，而血清GSH及GSSG/GSH可能被视为预防MiRAS发生的保护因素。
GSSG可能是MiRAS的潜在危险因素，GSH可能是保护因素，而GR可能在MiRAS的发病机制中不发挥重要作用。
