Courson Rémi, Bratash Oleksii, Maziz Ali, Desmet Cloé, Meza Ricardo Alvarado, Leroy Loïc, Engel Elodie, Buhot Arnaud, Malaquin Laurent, Leïchlé Thierry
LAAS-CNRS, Université de Toulouse, CNRS, 31400 Toulouse, France.
Université Grenoble Alpes, CNRS, CEA, IRIG, SyMMES, 38000 Grenoble, France.
Microsyst Nanoeng. 2023 Jul 4;9:85. doi: 10.1038/s41378-023-00559-3. eCollection 2023.
In this work, we introduce a polymer version of a previously developed silicon MEMS drop deposition tool for surface functionalization that consists of a microcantilever integrating an open fluidic channel and a reservoir. The device is fabricated by laser stereolithography, which offers the advantages of low-cost and fast prototyping. Additionally, thanks to the ability to process multiple materials, a magnetic base is incorporated into the cantilever for convenient handling and attachment to the holder of a robotized stage used for spotting. Droplets with diameters ranging from ∼50 µm to ∼300 µm are printed upon direct contact of the cantilever tip with the surface to pattern. Liquid loading is achieved by fully immersing the cantilever into a reservoir drop, where a single load results in the deposition of more than 200 droplets. The influences of the size and shape of the cantilever tip and the reservoir on the printing outcome are studied. As a proof-of-concept of the biofunctionalization capability of this 3D printed droplet dispenser, microarrays of oligonucleotides and antibodies displaying high specificity and no cross-contamination are fabricated, and droplets are deposited at the tip of an optical fiber bundle.
在这项工作中，我们介绍了一种聚合物版本的先前开发的用于表面功能化的硅微机电系统液滴沉积工具，该工具由集成了开放流体通道和储液器的微悬臂梁组成。该器件通过激光立体光刻制造，具有低成本和快速原型制作的优点。此外，由于能够加工多种材料，在悬臂梁中加入了磁性底座，便于操作并附着到用于点样的自动化平台的支架上。当悬臂梁尖端与待图案化表面直接接触时，可打印出直径范围从约50微米到约300微米的液滴。通过将悬臂梁完全浸入储液器液滴中来实现液体加载，单次加载可沉积200多个液滴。研究了悬臂梁尖端和储液器的尺寸及形状对打印结果的影响。作为这种3D打印液滴分配器生物功能化能力的概念验证，制备了显示出高特异性且无交叉污染的寡核苷酸和抗体微阵列，并将液滴沉积在光纤束尖端。
