BECN2-ATG14 卷曲螺旋相互作用对于 GPRASP1/GASP1 相关 GPCR 的内体溶酶体降解具有选择性的关键作用。
The potent BECN2-ATG14 coiled-coil interaction is selectively critical for endolysosomal degradation of GPRASP1/GASP1-associated GPCRs.
机构信息
Department of Applied Biology and Chemical Technology, State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hong Kong, P. R. China.
Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, the First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. China.
出版信息
Autophagy. 2023 Nov;19(11):2884-2898. doi: 10.1080/15548627.2023.2233872. Epub 2023 Jul 13.
AMBRA1 autophagy and beclin 1 regulator 1; ATG14 autophagy related 14; ATG5 autophagy related 5; ATG7 autophagy related 7; BECN1 beclin 1; BECN2 beclin 2; CC coiled-coil; CQ chloroquine CNR1/CB1R cannabinoid receptor 1 DAPI 4',6-diamidino-2-phenylindole; dCCD delete CCD; DRD2/D2R dopamine receptor D2 GPRASP1/GASP1 G protein-coupled receptor associated sorting protein 1 GPCR G-protein coupled receptor; ITC isothermal titration calorimetry; IP immunoprecipitation; KD knockdown; KO knockout; MAP1LC3/LC3 microtubule associated protein 1 light chain 3; NRBF2 nuclear receptor binding factor 2; OPRD1/DOR opioid receptor delta 1 PIK3C3/VPS34 phosphatidylinositol 3-kinase catalytic subunit type 3; PIK3R4/VPS15 phosphoinositide-3-kinase regulatory subunit 4; PtdIns3K class III phosphatidylinositol 3-kinase; PtdIns3P phosphatidylinositol-3-phosphate; RUBCN rubicon autophagy regulator; SQSTM1/p62 sequestosome 1; UVRAG UV radiation resistance associated; VPS vacuolar protein sorting; WT wild type.
AMBRA1 自噬和 beclin 1 调节因子 1；ATG14 自噬相关 14；ATG5 自噬相关 5；ATG7 自噬相关 7；BECN1 beclin 1；BECN2 beclin 2；CC 卷曲螺旋；CQ 氯喹；CNR1/CB1R 大麻素受体 1；DAPI 4',6-二脒基-2-苯基吲哚；dCCD 删除 CCD；DRD2/D2R 多巴胺受体 D2；GPRASP1/GASP1 G 蛋白偶联受体相关分拣蛋白 1；GPCR G 蛋白偶联受体；ITC 等温滴定量热法；IP 免疫沉淀；KD 敲低；KO 敲除；MAP1LC3/LC3 微管相关蛋白 1 轻链 3；NRBF2 核受体结合因子 2；OPRD1/DOR 阿片受体 delta 1；PIK3C3/VPS34 磷脂酰肌醇 3-激酶催化亚单位 3；PIK3R4/VPS15 磷酸肌醇-3-激酶调节亚基 4；PtdIns3K 类 III 磷脂酰肌醇 3-激酶；PtdIns3P 磷脂酰肌醇-3-磷酸；RUBCN rubicon 自噬调节因子；SQSTM1/p62 sequestosome 1；UVRAG UV 辐射抗性相关；VPS 液泡蛋白分选；WT 野生型。
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