J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, United States of America.
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida, United States of America.
PLoS One. 2023 Jul 7;18(7):e0280169. doi: 10.1371/journal.pone.0280169. eCollection 2023.
The spiny mouse (Acomys) is gaining popularity as a research organism due to its phenomenal regenerative capabilities. Acomys recovers from injuries to several organs without fibrosis. For example, Acomys heals full thickness skin injuries with rapid re-epithelialization of the wound and regeneration of hair follicles, sebaceous glands, erector pili muscles, adipocytes, and dermis without scarring. Understanding mechanisms of Acomys regeneration may uncover potential therapeutics for wound healing in humans. However, access to Acomys colonies is limited and primary fibroblasts can only be maintained in culture for a limited time. To address these obstacles, we generated immortalized Acomys dermal fibroblast cell lines using two methods: transfection with the SV40 large T antigen and spontaneous immortalization. The two cell lines (AcoSV40 and AcoSI-1) maintained the morphological and functional characteristics of primary Acomys fibroblasts, including maintenance of key fibroblast markers and ECM deposition. The availability of these cells will lower the barrier to working with Acomys as a model research organism, increasing the pace at which new discoveries to promote regeneration in humans can be made.
刺鼠（Acomys）因其出色的再生能力而成为一种受欢迎的研究生物。Acomys 在没有纤维化的情况下从多个器官的损伤中恢复。例如，Acomys 通过伤口的快速再上皮化和毛囊、皮脂腺、竖毛肌、脂肪细胞和真皮的再生，完全治愈全层皮肤损伤，而不会形成疤痕。了解 Acomys 再生的机制可能会发现人类伤口愈合的潜在治疗方法。然而，获得 Acomys 群体受到限制，原代成纤维细胞只能在培养中维持有限的时间。为了解决这些障碍，我们使用两种方法生成了永生化的 Acomys 真皮成纤维细胞系：SV40 大 T 抗原转染和自发永生化。这两个细胞系（AcoSV40 和 AcoSI-1）保持了原代 Acomys 成纤维细胞的形态和功能特征，包括维持关键成纤维细胞标志物和细胞外基质沉积。这些细胞的可用性将降低使用 Acomys 作为模型研究生物的障碍，加快促进人类再生的新发现的步伐。
