血清中乙肝e抗原的大小两种形式：用单克隆抗体的双位点夹心放射免疫分析法测定
Small and large forms of hepatitis B e antigen in the serum: determination by two-site sandwich radioimmunoassay with monoclonal antibodies.
作者信息
Baba K, Ise I, Aihara S, Kishimoto S, Tsuda F, Tachibana K, Miyakawa Y, Mayumi M
出版信息
Clin Exp Immunol. 1986 May;64(2):295-301.
Serum samples containing hepatitis B e antigen (HBeAg) were subjected to electrophoresis in agarose, and fast-migrating 'small' HBeAg and slow-migrating 'large' IgG-associated HBeAg were pooled separately. HBeAg of each category was determined by a two-site radioimmunoassay that sandwiched HBeAg between monoclonal antibody (anti-HBe) against one epitope of HBeAg, fixed on a solid support, and anti-HBe against another epitope, labelled with radioiodine. Eighteen sera in which small HBeAg dominated revealed activities of hepatitis B surface antigen-associated DNA polymerase significantly higher than 14 sera in which large HBeAg dominated (logarithm of ct/min, mean +/- s.e. 3.36 +/- 0.08 versus 2.14 +/- 0.11, P less than 0.01). Shift from small HBeAg to large HBeAg was observed, along with the disappearance of DNA polymerase, in the serum from two carriers who seroconverted to anti-HBe.
将含有乙肝e抗原（HBeAg）的血清样本在琼脂糖中进行电泳，快速迁移的“小分子”HBeAg和慢速迁移的“大分子”IgG相关HBeAg分别收集。每一类HBeAg通过双位点放射免疫测定法进行检测，该方法将HBeAg夹在固定于固相支持物上的针对HBeAg一个表位的单克隆抗体（抗-HBe）和用放射性碘标记的针对另一个表位的抗-HBe之间。18份以小分子HBeAg为主的血清中乙肝表面抗原相关DNA聚合酶的活性显著高于14份以大分子HBeAg为主的血清（每分钟计数的对数，平均值±标准误为3.36±0.08对2.14±0.11，P<0.01）。在两名血清转换为抗-HBe的携带者的血清中，观察到从小分子HBeAg向大分子HBeAg的转变，同时DNA聚合酶消失。
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