Mohd Kashim Mohd Izhar Ariff, Abdul Haris Alia Aryssa, Hasim Nur Asmadayana, Abd Mutalib Sahilah, Anuar Nurina
Research Centre of Sharia, Faculty of Islamic Studies, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia.
Institute of Islam Hadhari, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia.
Foods. 2022 Oct 17;11(20):3235. doi: 10.3390/foods11203235.
Meat culturing technology goes beyond laboratory research and materialises in the market. Nonetheless, this technology has raised concerns among Muslim consumers worldwide due to its medium, especially foetal bovine serum (FBS), which originates from blood. Thus, the aim of this research was to determine the status of cultured meat by detecting species-specific DNA of bovine serum as one of the media used during meat production. Polymerase chain reaction (PCR) analysis was conducted by targeting mitochondrial cytochrome oxidase II (COII) gene sequences, producing a 165 bp amplicon. The sequences of the primers used were Bovine-F, 5'-CAT CAT AGC AAT TGC CAT AGT CC-3' and Bovine-R, 5'-GTA CTA GTA GTA TTA GAG CTA GAA TTA G-3'. DNA extraction was conducted using a QIAGEN Blood and Tissue™ commercial kit. The presence study also included a literature review on the (transformation) concept in order to determine the status of cultured meat. The results revealed that bovine DNA was detected in all samples tested using PCR analysis. Therefore, (perfect transformation) does not occur due to the ability of PCR analysis to detect bovine DNA in FBS and is prohibited according to Shariah law.
肉类培养技术已超越实验室研究阶段并在市场中得以实现。尽管如此，这项技术因其培养基，尤其是源自血液的胎牛血清（FBS），引发了全球穆斯林消费者的担忧。因此，本研究的目的是通过检测作为肉类生产过程中使用的培养基之一的牛血清的物种特异性DNA，来确定培养肉的状况。通过靶向线粒体细胞色素氧化酶II（COII）基因序列进行聚合酶链反应（PCR）分析，产生一个165 bp的扩增子。所用引物的序列为Bovine-F，5'-CAT CAT AGC AAT TGC CAT AGT CC-3'和Bovine-R，5'-GTA CTA GTA GTA TTA GAG CTA GAA TTA G-3'。使用QIAGEN Blood and Tissue™商业试剂盒进行DNA提取。存在性研究还包括对（转化）概念的文献综述，以确定培养肉的状况。结果显示，使用PCR分析在所有测试样本中均检测到牛DNA。因此，由于PCR分析能够检测FBS中的牛DNA，（完美转化）并未发生，并且根据伊斯兰教法是被禁止的。
