Bektemur Guven, Bozali Kubra, Colak Sahin, Aktas Selman, Guler Eray Metin
Department of Public Health, University of Health Sciences Turkiye, Hamidiye Faculty of Medicine, Istanbul, Turkiye.
Department of Medical Biochemistry, University of Health Sciences Turkiye, Hamidiye Faculty of Medicine, Istanbul, Turkiye.
North Clin Istanb. 2023 Jun 20;10(3):335-340. doi: 10.14744/nci.2022.00947. eCollection 2023.
Severe inflammation and oxidative stress seen in COVID-19 patients cause cumulative antiviral effects, and serious inflammation increases tissue, oxidative damage, and DNA damage. Therefore, in this study, oxidative stress, DNA damage, and inflammation biomarkers were investigated in patients diagnosed with COVID-19.
In this study, blood samples were obtained from 150 Covid-19 patients diagnosed by polymerase chain reaction and 150 healthy volunteers with the same demographic characteristics. Total oxidant status (TOS), total antioxidant status (TAS), total thiol (TT), native thiol, and myeloperoxidase (MPO) activities were measured by photometric methods. The levels of tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6), which are inflammation markers, were measured by the ELISA method using commercial kits. The genotoxic effect was evaluated by Comet Assay.
The oxidative stress biomarkers; Disulfide, TOS, MPO, oxidative stress index, and IL-1β, IL-6, and TNF-α levels of inflammation biomarkers and the DNA damage in COVID-19 patients were increased (p<0.001), and the levels of TAS, TT, and NT In COVID-19 patients were decreased (p<0.001).
In COVID-19 patients, induced DNA damage, inflammation, and oxidative stress can guide the prognosis and treatment strategies of the disease.
新冠病毒疾病（COVID-19）患者中出现的严重炎症和氧化应激会产生累积抗病毒效应，严重炎症会增加组织、氧化损伤和DNA损伤。因此，在本研究中，对确诊为COVID-19的患者的氧化应激、DNA损伤和炎症生物标志物进行了研究。
在本研究中，从150名经聚合酶链反应诊断为COVID-19的患者和150名具有相同人口统计学特征的健康志愿者中采集血样。采用光度法测量总氧化剂状态（TOS）、总抗氧化剂状态（TAS）、总硫醇（TT）、天然硫醇和髓过氧化物酶（MPO）活性。使用商业试剂盒通过酶联免疫吸附测定法（ELISA）测量炎症标志物肿瘤坏死因子-α（TNF-α）、白细胞介素1β（IL-1β）和白细胞介素6（IL-6）的水平。通过彗星试验评估遗传毒性效应。
COVID-19患者的氧化应激生物标志物；二硫化物、TOS、MPO、氧化应激指数以及炎症生物标志物的IL-1β、IL-6和TNF-α水平以及DNA损伤均增加（p<0.001），而COVID-19患者的TAS、TT和NT水平降低（p<0.001）。
在COVID-19患者中，诱导的DNA损伤、炎症和氧化应激可指导该疾病的预后和治疗策略。
