Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
Amorepacific R&I Center, Yonggu-daero, Yongin, Republic of Korea.
J Microbiol Biotechnol. 2023 Oct 28;33(10):1317-1328. doi: 10.4014/jmb.2306.06014. Epub 2023 Jul 12.
Green tea (GT) polyphenols undergo extensive metabolism within gastrointestinal tract (GIT), where their derivatives compounds potentially modulate the gut microbiome. This biotransformation process involves a cascade of exclusive gut microbial enzymes which chemically modify the GT polyphenols influencing both their bioactivity and bioavailability in host. Herein, we examined the in vitro interactions between 37 different human gut microbiota and the GT polyphenols. UHPLC-LTQ-Orbitrap-MS/MS analysis of the culture broth extracts unravel that genera , and KACC11451 promoted C-ring opening reaction in GT catechins. In addition, also hydrolyzed catechin galloyl esters to produce gallic acid and pyrogallol, and also converted flavonoid glycosides to their aglycone derivatives. Biotransformation of GT polyphenols into derivative compounds enhanced their antioxidant bioactivities in culture broth extracts. Considering the effects of GT polyphenols on specific growth rates of gut bacteria, we noted that GT polyphenols and their derivate compounds inhibited most species in phylum Actinobacteria, Bacteroides, and Firmicutes except genus . The present study delineates the likely mechanisms involved in the metabolism and bioavailability of GT polyphenols upon exposure to gut microbiota. Further, widening this workflow to understand the metabolism of various other dietary polyphenols can unravel their biotransformation mechanisms and associated functions in human GIT.
绿茶（GT）多酚在胃肠道（GIT）中经历广泛的代谢，其衍生物化合物可能调节肠道微生物组。这个生物转化过程涉及一系列独特的肠道微生物酶，它们通过化学修饰 GT 多酚来影响其在宿主中的生物活性和生物利用度。在此，我们研究了 37 种不同的人类肠道微生物群与 GT 多酚之间的体外相互作用。UHPLC-LTQ-Orbitrap-MS/MS 分析培养肉汤提取物表明，属、和 KACC11451 促进了 GT 儿茶素的 C 环开环反应。此外，还水解儿茶素没食子酸酯生成没食子酸和焦性没食子酸，并将类黄酮糖苷转化为其糖苷配基衍生物。GT 多酚转化为衍生化合物增强了它们在培养肉汤提取物中的抗氧化生物活性。考虑到 GT 多酚对肠道细菌特定生长速率的影响，我们注意到 GT 多酚及其衍生物化合物抑制了厚壁菌门、拟杆菌门和Firmicutes 门中的大多数物种，除了属。本研究描绘了 GT 多酚在暴露于肠道微生物群时代谢和生物利用度的可能机制。此外，扩大这个工作流程以了解各种其他膳食多酚的代谢可以揭示它们在人类 GIT 中的生物转化机制和相关功能。
