Bordson B L, Ricci E, Dickey R P, Dunaway H, Taylor S N, Curole D N
Fertil Steril. 1986 Sep;46(3):466-9. doi: 10.1016/s0015-0282(16)49587-7.
A series of 401 therapeutic donor insemination (TDI) cycles was analyzed for determining the effect on fecundability of fresh versus cryopreserved semen, the number of inseminations per cycle, recipient age, and the reproductive health of the TDI recipient. We followed a protocol in which inseminations were performed during the periovulatory period determined by urinary luteinizing hormone surge or ultrasound-timed human chorionic gonadotropin injection. The minimum standard for insemination with fresh or cryopreserved semen was a total of 40 X 10(6) grade 3 sperm. We found no decreased fecundability when using frozen semen, rather than freshly ejaculated specimens. The number of inseminations per cycle did not affect the pregnancy outcome after TDI. The reproductive health of the recipient had a significant effect on the pregnancy rate. Fecundability was 15.6% for healthy women, as compared with 6.7% for women with a diagnosis of moderate, severe, or extensive endometriosis, pelvic adhesions, tubal disease, or ovulatory dysfunction. To avoid transmission of human T-lymphotropic virus type III from sperm donor to TDI recipient, we have recently utilized an exclusively frozen TDI program. With well-timed inseminations of 40 X 10(6) grade 3 motile sperm, we have been able to attain pregnancy rates similar to those previously obtained with fresh samples.
分析了401个治疗性供体人工授精（TDI）周期，以确定新鲜精液与冷冻精液、每个周期的授精次数、受体年龄以及TDI受体的生殖健康状况对受孕能力的影响。我们遵循了一项方案，即在通过尿促黄体生成素激增或超声定时人绒毛膜促性腺激素注射确定的排卵期进行授精。新鲜或冷冻精液授精的最低标准是总共40×10⁶个3级精子。我们发现使用冷冻精液而非新鲜射出的标本时，受孕能力并未降低。每个周期的授精次数不影响TDI后的妊娠结局。受体的生殖健康状况对妊娠率有显著影响。健康女性的受孕能力为15.6%，而诊断为中度、重度或广泛性子宫内膜异位症、盆腔粘连、输卵管疾病或排卵功能障碍的女性受孕能力为6.7%。为避免人类III型嗜T细胞病毒从精子供体传播给TDI受体，我们最近采用了专门的冷冻TDI方案。通过适时授精40×10⁶个3级活动精子，我们已能够获得与之前使用新鲜样本时相似的妊娠率。
