Richter M A, Haning R V, Shapiro S S
Fertil Steril. 1984 Feb;41(2):277-80. doi: 10.1016/s0015-0282(16)47604-1.
The efficacies of fresh versus cryopreserved semen in the treatment of male factor infertility by artificial insemination by donor (AID) semen were directly compared by using the patient as her own control. In any one cycle, either fresh or frozen semen was used. The type of semen preparation was randomly assigned for the first cycle and varied thereafter according to donor availability. The same donor was used for a given patient in six consecutive cycles. We treated 381 patients in this way. In 676 cycles fresh semen was used and 128 pregnancies were achieved. Fecundability, the chance of getting pregnant per cycle of exposure, was 18.9% with fresh semen. In 1200 cycles cryopreserved semen was used and 60 pregnancies occurred, for a fecundability of 5.0%. Therefore, in our clinic, fresh semen is more than three times as likely to induce pregnancy as frozen semen. The design that has been used in this therapeutic protocol provides a technique for internal quality control of the cryopreservation process and for the investigation of other variables potentially affecting the success rates with AID.
通过将患者自身作为对照，直接比较了新鲜精液与冷冻精液在供体人工授精（AID）治疗男性因素不孕症中的疗效。在任何一个周期中，要么使用新鲜精液，要么使用冷冻精液。精液制备类型在第一个周期随机分配，此后根据供体的可用性而有所不同。同一供体在连续六个周期中用于给定患者。我们用这种方法治疗了381名患者。在676个周期中使用了新鲜精液，实现了128次妊娠。新鲜精液的受孕能力，即每次暴露周期怀孕的机会，为18.9%。在1200个周期中使用了冷冻精液，发生了60次妊娠，受孕能力为5.0%。因此，在我们的诊所，新鲜精液诱导怀孕的可能性是冷冻精液三倍多。本治疗方案中使用的设计为冷冻保存过程的内部质量控制以及调查其他可能影响AID成功率的变量提供了一种技术。
