Zara Susi, Fioravanti Giulia, Ciuffreda Angelo, Annicchiarico Ciro, Quaresima Raimondo, Mastrangelo Filiberto
Department of Pharmacy, University G. D'Annunzio of Chieti-Pescara, 66100 Chieti, Italy.
Department of Physical and Chemical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Materials (Basel). 2023 Jun 22;16(13):4530. doi: 10.3390/ma16134530.
The use of ytterbium laser to obtain colored titanium surfaces is a suitable strategy to improve the aesthetic soft tissue results and reduce implant failures in oral rehabilitation. To investigate the relationship between novel laser-colored surfaces and peri-implant soft tissues, Human Gingival Fibroblasts (HGFs) were cultured onto 12 colored titanium grade 1 light fuchsia, dark fuchsia, light gold, and dark gold disks and their viability (MTT Assay), cytotoxicity (lactate dehydrogenase release), and collagen I secretion were compared to the machined surface used as control. Optical and electronic microscopies showed a HGF growth directly correlated to the roughness and wettability of the colored surfaces. A higher viability percentage on dark fuchsia (125%) light gold (122%), and dark gold (119%) samples with respect to the machined surface (100%) was recorded. All specimens showed a statistically significant reduction of LDH release compared to the machined surface. Additionally, a higher collagen type I secretion, responsible for an improved adhesion process, in light fuchsia (3.95 μg/mL) and dark gold (3.61 μg/mL) compared to the machined surface (3.59 μg) was recorded. The in vitro results confirmed the innovative physical titanium improvements due to laser treatment and represent interesting perspectives of innovation in order to ameliorate aesthetic dental implant performance and to obtain more predictable osteo and perio-osteointegration long term implant prognosis.
使用镱激光处理钛表面以获得彩色涂层是一种合适的策略，可改善口腔修复中软组织的美学效果并减少种植体失败。为了研究新型激光彩色表面与种植体周围软组织之间的关系，将人牙龈成纤维细胞（HGFs）培养在12个彩色的1级钛盘上，颜色分别为浅紫红色、深紫红色、浅金色和深金色，将其活力（MTT法）、细胞毒性（乳酸脱氢酶释放）和I型胶原蛋白分泌与作为对照的机械加工表面进行比较。光学和电子显微镜显示HGF的生长与彩色表面的粗糙度和润湿性直接相关。记录到深紫红色（125%）、浅金色（122%）和深金色（119%）样品相对于机械加工表面（100%）具有更高的活力百分比。与机械加工表面相比，所有标本的乳酸脱氢酶释放均有统计学显著降低。此外，记录到浅紫红色（3.95μg/mL）和深金色（3.61μg/mL）的I型胶原蛋白分泌量高于机械加工表面（3.59μg），这有助于改善黏附过程。体外结果证实了激光处理对钛进行的创新性物理改进，并代表了有趣的创新前景，以改善种植牙的美学性能，并获得更可预测的骨整合和牙周骨整合，实现长期种植预后。
