在果实成熟过程中进行基因家族的全基因组研究：进化和表达网络特征。
Genome-Wide Investigation of Gene Family in : Evolution and Expression Network Profiles during Fruit Ripening.
机构信息
Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China.
Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China.
出版信息
Int J Mol Sci. 2023 Jun 22;24(13):10516. doi: 10.3390/ijms241310516.
β-amylase proteins (BAM) are important to many aspects of physiological process such as starch degradation. However, little information was available about the genes in , an important tropical fruit. Seven genes containing the conservative domain of glycoside hydrolase family 14 (PF01373) were identified with genome, and these genes can be divided into four groups. Subcellular localization analysis revealed that and were located in the chloroplast, and was located in the cell membrane and the chloroplast. The belonging to Subfamily I contribute to starch degradation have the higher expression than those belonging to Subfamily II. The analysis of the expression showed that may function in the whole fruit ripening process, and may be important to starch degradation in other organs. Temperature and ethylene affect the expression of major genes in Subfamily I during fruit ripening. These expressions and subcellular localization results indicating β-amylase play an important role in starch degradation.
β-淀粉酶蛋白（BAM）对许多生理过程都很重要，如淀粉降解。然而，关于重要的热带水果中的基因，信息却很少。通过基因组鉴定出了 7 个含有糖苷水解酶家族 14 保守结构域（PF01373）的基因，这些基因可以分为 4 组。亚细胞定位分析表明和定位于叶绿体，而定位于细胞膜和叶绿体。属于 I 亚家族的参与淀粉降解的与属于 II 亚家族的相比，表达量更高。分析表明，可能在整个果实成熟过程中发挥作用，而可能对其他器官中的淀粉降解很重要。温度和乙烯在果实成熟过程中影响 I 亚家族中主要基因的表达。这些表达和亚细胞定位结果表明β-淀粉酶在淀粉降解中起着重要作用。
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