Pharmaceutical Chemistry Research Group, Institut Químic de Sarrià, Universitat Ramon Llull, 08017 Barcelona, Spain.
Int J Mol Sci. 2023 Jul 3;24(13):11016. doi: 10.3390/ijms241311016.
The use of animal testing in the cosmetic industry is already prohibited in more than 40 countries, including those of the EU. The pressure for it to be banned worldwide in the future is increasing, so the need for animal alternatives is of great interest today. In addition, using animals and humans in scientific research is ethically reprehensible. This study aimed to prove some of the anti-aging properties of elastin (EL), hydrolyzed collagen (HC), and two vegan collagen-like products (Veg Col) in a tri-layered chitosan membrane that was ionically crosslinked with sodium tripolyphosphate (TPP). In the first approach, as a way of representing different layers of a biological system, such as the epidermis and the two dermis sublayers, EL, HC, or Veg Col were independently introduced into the two inner layers (2L). Their effects were compared with those of their introduction into three layers (3L). Different experiments were performed on the membrane to test its elasticity, hydration, moisture retention, and pore reduction at different concentrations of EL, HC, and Veg Col, and the results were normalized vs. a blank membrane. This new alternative to animal or human testing can be suitable for proving certain efficacy claims for active ingredients or products in the pharmaceutical, nutritional, and cosmetic fields.
在化妆品行业中使用动物测试已经在 40 多个国家被禁止，包括欧盟国家。未来在全球范围内禁止使用动物测试的压力越来越大，因此，对动物替代品的需求在今天具有很大的意义。此外，在科学研究中使用动物和人类在伦理上是应受谴责的。本研究旨在证明弹性蛋白（EL）、水解胶原蛋白（HC）和两种植物性胶原蛋白样产品（Veg Col）在与三聚磷酸钠（TPP）离子交联的三层壳聚糖膜中的一些抗衰老特性。在第一种方法中，作为代表生物系统不同层的一种方式，如表皮和两个真皮亚层，EL、HC 或 Veg Col 被独立引入两个内层（2L）。将它们的效果与它们引入三层（3L）的效果进行比较。在不同浓度的 EL、HC 和 Veg Col 下，对膜进行了不同的实验，以测试其弹性、水合作用、保水能力和孔径减小，并将结果与空白膜进行归一化。这种替代动物或人体测试的新方法可以适用于证明药物、营养和化妆品领域中活性成分或产品的某些功效主张。
