Key Laboratory of Poyang Lake Environment and Resource Utilization, Ministry of Education, School of Life Sciences, Nanchang University, Nanchang 330031, China.
Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666300, China.
Int J Mol Sci. 2024 Sep 2;25(17):9538. doi: 10.3390/ijms25179538.
R.Br. is an epiphytic orchid genus with significant horticultural and ornamental value. Previous molecular studies expanded including some members from five other genera. However, the interspecific relationships of this recently radiated genus have remained unclear based on several DNA markers until now. In this study, the complete plastome has been used to infer the phylogenetic relationships of . The five newly obtained plastomes ranged from 146,340 bp to 149,273 bp in length, with a GC content ranging from 36.5% to 36.7%. The five plastomes contained 74 protein-coding genes (CDSs), 38 tRNAs, and 8 rRNAs, and their genes underwent loss or pseudogenization. Comparative plastome analyses of 13 species revealed high conservation in terms of genome size, structure, and gene order, except for a large inversion from to in . Moreover, six CDSs and five non-CDSs were selected as candidate DNA barcodes. Our phylogenetic analyses demonstrated that is a monophyletic group with high supporting values based on five different datasets (complete plastome with one IR, 68 CDSs, LSC, five hypervariable non-CDSs, and six hypervariable CDSs), while the phylogenetic relationships among species were fully resolved based on the complete plastome with one IR dataset. Our results confirmed that the complete plastome has a great power in resolving the phylogenetic relationships of recently radiated lineages.
R.Br. 是一个具有重要园艺和观赏价值的附生兰属。以前的分子研究已经扩展到包括来自另外五个属的一些成员。然而，基于几个 DNA 标记，这个最近辐射的属的种间关系至今仍不清楚。在这项研究中，完整的质体基因组被用来推断 的系统发育关系。新获得的五个质体基因组长度从 146340bp 到 149273bp 不等，GC 含量从 36.5%到 36.7%不等。这五个质体基因组包含 74 个蛋白编码基因（CDS）、38 个 tRNA 和 8 个 rRNA，其 基因发生了丢失或假基因化。对 13 个 种的比较质体分析表明，除了在 中从 到 的大规模倒位外，基因组大小、结构和基因顺序高度保守。此外，六个 CDS 和五个非 CDS 被选为候选 DNA 条形码。我们的系统发育分析表明， 是一个单系群，基于五个不同数据集（完整的质体基因组与一个 IR、68 个 CDS、LSC、五个高变非 CDS 和六个高变 CDS）具有高支持值，而基于完整的质体基因组与一个 IR 数据集的种间关系得到了完全解决。我们的结果证实，完整的质体基因组在解决最近辐射谱系的系统发育关系方面具有很大的力量。
