Marine Research Institute, Klaipeda University, H. Manto Street 84, LT-92294 Klaipeda, Lithuania.
Sensors (Basel). 2023 Jul 3;23(13):6129. doi: 10.3390/s23136129.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), has emerged as a serious threat to human health worldwide. The effective disinfection of surfaces contaminated with SARS-CoV-2 may help prevent its spread. The aim of this study is to determine the duration required for viral RNA elimination by 222 nm far ultraviolet light using RT-qPCR as a tool. This study investigated the effect of 222 nm UVC irradiation on SARS-CoV-2 RNA in an in vitro experiment. The results showed that the copy number of SARS-CoV-2 RNA did not change even after 300 s of 222 nm UVC irradiation at 0.1 mW/cm, but extending the exposure to more than 600 s reduced the number of copies of SARS-CoV-2 virus significantly. However, to fully validate the results and enhance the robustness of the findings, it is crucial to increase the number of samples analyzed in future experiments.
严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）可引起 2019 年冠状病毒病（COVID-19），已成为全球范围内人类健康的严重威胁。对受到 SARS-CoV-2 污染的表面进行有效消毒，可能有助于预防其传播。本研究旨在通过实时荧光定量聚合酶链反应（RT-qPCR）确定使用 222nm 远紫外线消除病毒 RNA 所需的时间。本研究通过体外实验调查了 222nmUVC 照射对 SARS-CoV-2RNA 的影响。结果表明，即使在 0.1mW/cm2 的 222nmUVC 照射 300s 后，SARS-CoV-2RNA 的拷贝数也没有变化，但延长暴露时间超过 600s 会显著降低 SARS-CoV-2 病毒的拷贝数。然而，为了充分验证结果并增强研究结果的稳健性，未来的实验中增加分析的样本数量至关重要。
