对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）阴性患者进行人鼻病毒的频繁检测和基因分型：一项来自伊朗南部的研究。

Frequent detection and genotyping of human rhinovirus in SARS-CoV-2 negative patients; a study from south of Iran.

作者信息

Darabi Amirhossein, Sarshari Behrang, Vahdat Katayoun, Mousavi Seyed Alireza, Keshavarz Mohsen

机构信息

The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.

Iranian Research Center for HIV/AIDS, Iranian Institute for Reduction of High-Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Microbiol. 2023 Jun;15(3):462-467. doi: 10.18502/ijm.v15i3.12908.

DOI:10.18502/ijm.v15i3.12908

PMID:37448684

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10336278/

Abstract

BACKGROUND AND OBJECTIVES

Human rhinovirus (HRV), a major cause of common cold, was associated to the hospitalization of children and adults. This cross-sectional study aimed to determine the prevalence, and genotype distribution of HRV in the patients with mild to severe respiratory infections who were negative for SARS-Cov-2.

MATERIALS AND METHODS

Nasopharyngeal swab specimens (n = 356) from the patients aged 29 days to 82 years, received for the respiratory virus detection from January to December 2021, were analyzed for human rhinovirus (HRV) by RT-PCR. As a final step, genotyping was performed on obtained sequences.

RESULTS

A total of 37 HRV infections were identified (37/356, 10%). The highest rates of positive HRV tests were observed in February (21.6%), and January (18.9%), compared with June and August (0%). HRV-positive cases mainly appeared in winter. Among the age groups, those 2-<5 years of age had the highest detection rate (21%), however, those >55 years of age had the lowest detection rate (3%). Among HRV-positive samples, 30 (81%) were identified as type HRV-A, 5 (13.5%) as HRV-B, and 2 (5.5%) as HRV-C.

CONCLUSION

Our results suggested that HRV frequency gradually decreased with the age of patients which is more active in Iran, especially in the cold months.

摘要

背景与目的

人鼻病毒（HRV）是普通感冒的主要病因，与儿童和成人住院有关。本横断面研究旨在确定新型冠状病毒（SARS-CoV-2）检测呈阴性的轻至重度呼吸道感染患者中HRV的流行率和基因型分布。

材料与方法

收集2021年1月至12月期间年龄在29天至82岁之间、因呼吸道病毒检测而接受检测的患者的鼻咽拭子标本（n = 356），采用逆转录聚合酶链反应（RT-PCR）分析人鼻病毒（HRV）。最后，对获得的序列进行基因分型。

结果

共鉴定出37例HRV感染（37/356，10%）。与6月和8月（0%）相比，2月（21.6%）和1月（18.9%）的HRV检测阳性率最高。HRV阳性病例主要出现在冬季。在各年龄组中，2至<5岁年龄组的检出率最高（21%），而>55岁年龄组的检出率最低（3%）。在HRV阳性样本中，30例（81%）被鉴定为HRV-A型，5例（13.5%）为HRV-B型，2例（5.5%）为HRV-C型。