Singh Rajat Kumar, Reuber Emelie E, Bruno Mariolina, Netea Mihai G, Seeberger Peter H
Department of Biomolecular System, Max Planck Institute of Colloids and Interfaces 14476 Potsdam Germany
Institute of Chemistry and Biochemistry, Freie Universität Berlin 14195 Berlin Germany.
Chem Sci. 2023 Jun 23;14(27):7559-7563. doi: 10.1039/d3sc01242e. eCollection 2023 Jul 12.
() is an emerging multidrug-resistant fungal pathogen that represents a significant public health challenge as it can spread rapidly and result in high mortality rates. The mannans on the cell surface are potent immunogens and attractive targets for developing a glycoconjugate vaccine. We synthesized the oligosaccharides resembling cell surface mannans of and printed them onto microarray slides that were used to screen plasma from mice infected with . IgM antibodies in mouse plasma recognize the β-1,2 linkage present in surface mannans. Disaccharide 19 emerged from glycan array screening as a lead for developing a vaccine against , as the majority of patient plasma samples showed antibodies against this glycan. The synthetic oligosaccharides can be used for the early detection of infections.
（）是一种新兴的多重耐药真菌病原体，它能迅速传播并导致高死亡率，对公共卫生构成重大挑战。细胞表面的甘露聚糖是有效的免疫原，也是开发糖缀合物疫苗的有吸引力的靶点。我们合成了类似于（）细胞表面甘露聚糖的寡糖，并将其打印到微阵列载玻片上，用于筛选感染（）的小鼠的血浆。小鼠血浆中的IgM抗体识别（）表面甘露聚糖中存在的β-1,2连接。二糖19从聚糖阵列筛选中脱颖而出，成为开发抗（）疫苗的先导，因为大多数患者血浆样本显示出针对这种聚糖的抗体。合成的寡糖可用于（）感染的早期检测。
