Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, USA.
System Biology Institute, Yale University, West Haven, Connecticut, USA.
Immunol Rev. 2023 Nov;320(1):199-216. doi: 10.1111/imr.13241. Epub 2023 Jul 14.
CRISPR technology has transformed multiple fields, including cancer and immunology. CRISPR-based gene editing and screening empowers direct genomic manipulation of immune cells, opening doors to unbiased functional genetic screens. These screens aid in the discovery of novel factors that regulate and reprogram immune responses, offering novel drug targets. The engineering of immune cells using CRISPR has sparked a transformation in the cellular immunotherapy field, resulting in a multitude of ongoing clinical trials. In this review, we discuss the development and applications of CRISPR and related gene editing technologies in immune cells, focusing on functional genomics screening, gene editing-based cell therapies, as well as future directions in this rapidly advancing field.
CRISPR 技术已经改变了多个领域，包括癌症和免疫学。基于 CRISPR 的基因编辑和筛选使免疫细胞的直接基因组操作成为可能，为无偏功能遗传筛选开辟了道路。这些筛选有助于发现调节和重编程免疫反应的新因素，提供新的药物靶点。使用 CRISPR 工程化免疫细胞已经引发了细胞免疫治疗领域的变革，导致了大量正在进行的临床试验。在这篇综述中，我们讨论了 CRISPR 及相关基因编辑技术在免疫细胞中的发展和应用，重点介绍了功能基因组筛选、基于基因编辑的细胞疗法，以及这个快速发展领域的未来方向。
