Gao Xiaoyu, Li Yao, Nie Guangjun, Zhao Xiao
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, 11 Beiyitiao, Zhongguancun, Beijing, 100190, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Bio Protoc. 2023 Jul 5;13(13):e4774. doi: 10.21769/BioProtoc.4774.
The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding.
定制化肿瘤mRNA疫苗的快速展示与递送方法存在局限性。在此，通过对一种RNA结合蛋白L7Ae进行表面工程改造，将细菌衍生的外膜囊泡（OMV）用作mRNA递送平台。OMV-L7Ae可通过L7Ae-boxC/D结合快速吸附盒C/D序列标记的mRNA抗原，并将其递送至HEK-293T细胞和树突状细胞中。该平台提供了一种不同于脂质纳米颗粒（LNP）的mRNA递送技术，用于个性化mRNA肿瘤疫苗接种，且具有适用于快速制备针对多种肿瘤抗原的个性化mRNA肿瘤疫苗的策略。关键特性 通过L7Ae-boxC/D结合将OMV用作mRNA递送平台。
