Laboratório de Estudos Genômicos, Departamento de Biologia, Instituto de Biociências Letras E Ciências Exatas (IBILCE), Universidade Estadual Paulista (Unesp), São José Do Rio Preto, Brazil.
The Rockefeller University, 1230 York Ave, Manhattan, New York, NY, 10065, USA.
Braz J Microbiol. 2023 Sep;54(3):1421-1431. doi: 10.1007/s42770-023-01053-8. Epub 2023 Jul 17.
Zika virus (ZIKV) is an arbovirus maintained in nature in two distinct cycles of transmission: urban and sylvatic. Each cycle includes specific vertebrate and invertebrate hosts, and through alternate infections, a conserved consensus sequence is maintained that might vary depending on the cycle. The current study aimed to investigate the ability of ZIKV and ZIKV to maintain an infectious cycle by alternating passages in cells mimicking the urban (UC) and semi-sylvatic (SC) cycles. The complete genome of the original inoculum and the last passages for each cycle were sequenced by Sanger. Ten passages were performed, as planned, for ZIKV UC, ZIKV SC, and ZIKV SC. ZIKV SC showed significant variation in viral titers along the passages, suggesting that the virus is not well adapted to the non-human primate host. ZIKV passage in UC was abrogated in the third passage, showing the inability of the African lineage to sustain cycles in human cells, suggesting a low capacity to establish an urban cycle. Several mutations were found in both strains along the passages, but not occurring at equivalent positions. Further studies are needed to elucidate whether any of these specific mutations affect viral fitness. ZIKV strains behave differently in artificial transmission cycles in vitro: Brazilian ZIKV was able to establish urban and semi-sylvatic cycles in vitro. African ZIKV proved unable to cycle among human and mosquito cells and is compatible only with the semi-sylvatic cycle. The main mutations arose in the NS2A region after artificial transmission cycles for both ZIKV strains but not at equivalent positions.
寨卡病毒（ZIKV）在两种不同的传播循环中维持自然发生：城市和森林。每个循环包括特定的脊椎动物和无脊椎动物宿主，通过交替感染，维持保守的共识序列，该序列可能因循环而异。本研究旨在研究 ZIKV 和 ZIKV 通过交替感染模拟城市（UC）和半森林（SC）循环的细胞来维持感染循环的能力。使用 Sanger 测序对原始接种物的完整基因组和每个循环的最后传代物进行测序。按计划进行了 10 次传代，用于 ZIKV UC、ZIKV SC 和 ZIKV SC。ZIKV SC 在传代过程中的病毒滴度发生了显著变化，表明该病毒不能很好地适应非人类灵长类宿主。ZIKV 在 UC 中的传代在第三传代中被阻断，表明非洲谱系不能在人类细胞中维持循环，这表明建立城市循环的能力较低。在两种菌株的传代过程中发现了几个突变，但不是在等效位置。需要进一步的研究来阐明这些特定突变是否会影响病毒适应性。ZIKV 株在体外人工传播循环中表现出不同的行为：巴西 ZIKV 能够在体外建立城市和半森林循环。非洲 ZIKV 被证明无法在人类和蚊子细胞之间循环，仅与半森林循环兼容。在两种 ZIKV 株的人工传播循环后，主要突变出现在 NS2A 区域，但不在等效位置。
