Kay D B, Cambier J L, Wheeless L L
J Histochem Cytochem. 1979 Jan;27(1):329-34. doi: 10.1177/27.1.374597.
Imaging in flow has been valuable in investigating discrepancies in flow cell measurements due to cell orientation and flow dynamics. This paper discusses optical consideration in flow imaging, slit and full field imaging systems and various cell motion arresting techniques from the standpoint of image plane exposure and suitable detector choices. It concludes with an explanation of the slit-imaging techniques employed in a multidimensional slit-scan flow system and slit-scan correlation system.
流动成像在研究由于细胞取向和流动动力学导致的流动池测量差异方面具有重要价值。本文从图像平面曝光和合适的探测器选择的角度讨论了流动成像中的光学考量、狭缝和全场成像系统以及各种细胞运动抑制技术。文章最后解释了多维狭缝扫描流动系统和狭缝扫描相关系统中所采用的狭缝成像技术。