Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, National and Local Joint Engineering Research Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, P. R. China.
Analyst. 2023 Aug 7;148(16):3791-3797. doi: 10.1039/d3an00829k.
The fabrication of electroactive nanostructures with high electron concentration and specific electron transport is crucial for electrochemical sensing. In this study, a plasmon-enhanced electrochemical sensor has been developed for the detection of extracellular hydrogen peroxide (HO) from cancer cells, utilizing Pd-tipped Au nanobipyramids (PTA NBPs) as the electrocatalysts. Plasmonic PTA NBPs were synthesized by depositing Pd nanoparticles onto the tips of Au nanobipyramids (Au NBPs). Under excitation of localized surface plasmon resonance (LSPR), the PTA NBPs generate high-energy electron-hole pairs (e/h) on their surface. The generated electrons (e) significantly enhance the electrochemical reduction of HO. Based on this, a plasmon-enhanced HO electrochemical sensor is constructed with high sensitivity (986.57 μA mM cm), low detection limit (0.02 μM), wide linear range (0.1 μM to 980 μM), and good stability and repeatability. Moreover, this sensor also enables the measurement of extracellular HO derived from cancer cells (MCF-7), highlighting its potential applications in cellular biology and biomedical research.
制备具有高电子浓度和特定电子传输能力的电活性纳米结构对于电化学生物传感至关重要。在这项研究中，利用 Pd 修饰的 Au 纳米双锥（PTA NBPs）作为电催化剂，开发了一种等离子体增强电化学传感器，用于检测癌细胞外的过氧化氢（HO）。等离子体 PTA NBPs 通过在 Au 纳米双锥（Au NBPs）的尖端沉积 Pd 纳米颗粒来合成。在局域表面等离子体共振（LSPR）的激发下，PTA NBPs 在其表面产生高能电子-空穴对（e/h）。产生的电子（e）显著增强了 HO 的电化学还原。基于此，构建了一种具有高灵敏度（986.57 μA mM cm）、低检测限（0.02 μM）、宽线性范围（0.1 μM 至 980 μM）和良好稳定性和重现性的等离子体增强 HO 电化学生物传感器。此外，该传感器还能够测量源自癌细胞（MCF-7）的细胞外 HO，突出了其在细胞生物学和生物医学研究中的潜在应用。
