Shin Minkyu, Yoon Jinho, Yi Chanyong, Lee Taek, Choi Jeong-Woo
Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 04107, Korea.
Department of Chemical Engineering, Kwangwoon University, Wolgye-dong, Nowon-gu, Seoul 01899, Korea.
Nanomaterials (Basel). 2019 Jul 26;9(8):1076. doi: 10.3390/nano9081076.
An electrochemical flexible biosensor composed of gold (Au), molybdenum disulfide nanoparticles (MoS NPs), and Au (Au/MoS/Au nanolayer) on the polyethylene terephthalate (PET) substrate is developed to detect envelope glycoprotein GP120 (gp120), the surface protein of HIV-1. To fabricate the nanolayer on the PET substrate, Au is sputter coated on the flexible PET substrate and MoS NPs are spin coated on Au, which is sputter coated once again with Au. The gp120 antibody is then immobilized on this flexible electrode through cysteamine (Cys) modified on the surface of the Au/MoS/Au nanolayer. Fabrication of the biosensor is verified by atomic force microscopy, scanning electron microscopy, and cyclic voltammetry. A flexibility test is done using a micro-fatigue tester. Detection of the gp120 is measured by square wave voltammetry. The results indicate that the prepared biosensor detects 0.1 pg/mL of gp120, which is comparable with previously reported gp120 biosensors prepared even without flexibility. Therefore, the proposed biosensor supports the development of a nanomaterial-based flexible sensing platform for highly sensitive biosensors with flexibility for wearable device application.
开发了一种由金（Au）、二硫化钼纳米颗粒（MoS NPs）和聚对苯二甲酸乙二醇酯（PET）基板上的金（Au/MoS/Au纳米层）组成的电化学柔性生物传感器，用于检测HIV-1表面蛋白包膜糖蛋白GP120（gp120）。为了在PET基板上制备纳米层，将金溅射涂覆在柔性PET基板上，然后将MoS NPs旋涂在金上，再用金进行一次溅射涂覆。然后通过在Au/MoS/Au纳米层表面修饰的半胱胺（Cys）将gp120抗体固定在该柔性电极上。通过原子力显微镜、扫描电子显微镜和循环伏安法验证了生物传感器的制备。使用微疲劳测试仪进行了柔韧性测试。通过方波伏安法测量gp120的检测。结果表明，所制备的生物传感器能够检测到0.1 pg/mL的gp120，这与之前报道的即使没有柔韧性的gp120生物传感器相当。因此，所提出的生物传感器支持了基于纳米材料的柔性传感平台的开发，该平台用于具有可穿戴设备应用灵活性的高灵敏度生物传感器。
