Medical Research Center, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, 317000, Zhejiang, China.
Key Laboratory of Minimally Invasive Techniques & Rapid Rehabilitation of Digestive System Tumor of Zhejiang Province, Linhai, Zhejiang, China.
Virol J. 2023 Jul 19;20(1):156. doi: 10.1186/s12985-023-02125-9.
Human papillomavirus (HPV) 33 belongs to the Alphapapillomavirus 9 (α-9 HPV) species group, which also contains types 16, 31, 35, 52, 58 and 67. The purpose of this study was to investigate the genetic variations of HPV33 and to explore its carcinogenicity among women in Taizhou, Southeast China.
Exfoliated cervical cells were collected for HPV genotyping. Only single HPV33 infection cases were selected, and their E6 and E7 genes were sequenced using the ABI 3730xl sequencer and then analysed using MEGA X.
From 2014 to 2020, a total of 185 single HPV33-positive specimens were successfully amplified. We obtained 15 distinct HPV33 E6/E7 variants, which were published in GenBank under accession numbers OQ672665-OQ672679. Phylogenetic analysis revealed that all HPV33 E6/E7 variants belonged to lineage A, of which 75.7% belonged to lineage A1. Compared with CIN1, the proportion of sublineage A1 in CIN2/3 was higher, but there was no significant difference (76.5% vs. 80.6%, P > 0.05). Altogether, 20 single nucleotide substitutions were identified, of which 6 were novel substitutions, including T196G (C30G), A447T, G458T (R117L), G531A, A704A, and C740T. In addition, no significant trends were observed between the nucleotide substitutions of HPV33 E6/E7 variants and the risk of cervical lesions.
This study provides the most comprehensive data on genetic variations, phylogenetics and carcinogenicity of HPV33 E6/E7 variants in Southeast China to date. The data confirmed that cervical lesions among women in Taizhou are attributable to HPV33, which may be due to the high infection rate of sublineage A1 in the population.
人乳头瘤病毒（HPV）33 属于阿尔法乳多空病毒 9（α-9 HPV）种组，该组还包含 16、31、35、52、58 和 67 型。本研究旨在调查 HPV33 的遗传变异，并探讨其在中国东南部台州地区女性中的致癌性。
收集宫颈脱落细胞进行 HPV 基因分型。仅选择单纯 HPV33 感染病例，使用 ABI 3730xl 测序仪对其 E6 和 E7 基因进行测序，并使用 MEGA X 进行分析。
2014 年至 2020 年，共成功扩增了 185 例单纯 HPV33 阳性标本。我们获得了 15 种不同的 HPV33 E6/E7 变体，这些变体已在 GenBank 中以 OQ672665-OQ672679 的登录号发表。系统进化分析表明，所有 HPV33 E6/E7 变体均属于谱系 A，其中 75.7%属于谱系 A1。与 CIN1 相比，CIN2/3 中小谱系 A1 的比例更高，但差异无统计学意义（76.5%比 80.6%，P>0.05）。共发现 20 个单核苷酸替换，其中 6 个为新的替换，包括 T196G（C30G）、A447T、G458T（R117L）、G531A、A704A 和 C740T。此外，HPV33 E6/E7 变体的核苷酸替换与宫颈病变的风险之间没有明显的趋势。
本研究提供了迄今为止中国东南部 HPV33 E6/E7 变体遗传变异、系统发育和致癌性最全面的数据。数据证实，台州地区女性的宫颈病变归因于 HPV33，这可能是由于该人群中 A1 亚谱系的高感染率所致。
