Yan Shujin, Wang Dong, Zhang Liang, Gan Tian, Yao Huan, Zhu Hui, He Yiman, Yang Ke
Ministry of Education Key Laboratory of Child Development and Disorders, Pediatric Research Institute, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, Children's Hospital of Chongqing Medical University, Chongqing, China.
Department of Ultrasound, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Front Bioeng Biotechnol. 2023 Jul 4;11:1226426. doi: 10.3389/fbioe.2023.1226426. eCollection 2023.
Poly (lactic-co-glycolic acid)-based nanoparticles (PLGA NPs) have been widely used as the carrier for sustainable drug delivery. However, the drug release from the NPs was usually incomplete and uncontrollable. Herein, a low intensity pulsed ultrasound (LIPUS) assisted SDF-1/BMP-2@nanoparticles (S/B@NPs) system was fabricated to facilitate stem cell recruitment-osteogenesis for periodontal bone regeneration. In this work, S/B@NPs were prepared with double-emulsion synthesis method. Then the S/B release profile from NPs was evaluated with or without low intensity pulsed ultrasound treatment. Afterwards, the stem cell recruiting and osteoinductive capacities of LIPUS-S/B@NPs were detected with human periodontal ligament cells (hPDLCs) and in a rat periodontal bone defect model. The results indicated that S/B@NPs were successfully prepared and LIPUS could effectively regulate the release of S/B and increase their final releasing amount. Moreover, LIPUS-S/B@NPs system significantly promoted hPDLCs migrating and osteogenesis and recruiting rBMSCs to the rat periodontal defect and facilitated bone regeneration . Our LIPUS assisted S/B@NPs system can effectively facilitate stem cell recruitment and periodontal bone regeneration. Considering its reliable safety and therapeutic effect on bone fracture, LIPUS, as an adjuvant therapy, holds great potential in the regulation of drug delivery systems for bone healing.
聚乳酸-羟基乙酸共聚物纳米颗粒（PLGA NPs）已被广泛用作可持续药物递送的载体。然而，纳米颗粒的药物释放通常不完整且不可控。在此，构建了一种低强度脉冲超声（LIPUS）辅助的SDF-1/BMP-2@纳米颗粒（S/B@NPs）系统，以促进干细胞募集和成骨作用，用于牙周骨再生。在这项工作中，采用双乳液合成法制备了S/B@NPs。然后评估有无低强度脉冲超声处理时纳米颗粒中S/B的释放情况。之后，用人牙周膜细胞（hPDLCs）并在大鼠牙周骨缺损模型中检测LIPUS-S/B@NPs的干细胞募集和骨诱导能力。结果表明，成功制备了S/B@NPs，LIPUS可有效调节S/B的释放并增加其最终释放量。此外，LIPUS-S/B@NPs系统显著促进hPDLCs迁移和成骨，将rBMSCs募集到大鼠牙周缺损处并促进骨再生。我们的LIPUS辅助S/B@NPs系统可有效促进干细胞募集和牙周骨再生。鉴于其可靠的安全性和对骨折的治疗效果，LIPUS作为一种辅助治疗方法，在调节骨愈合药物递送系统方面具有巨大潜力。
