在用聚（dA）·聚（dT）连接法将噬菌体MS2 DNA拷贝插入质粒pBR322 DNA构建的分子嵌合体中鉴定可转座元件IS1。 - Suppr

Suppr

超能文献

Identification of the translocatable element IS1 in a molecular chimera constructed with plasmid pBR322 DNA into which a bacteriophage MS2 DNA copy was inserted by the poly(dA).poly(dT) linker method.

作者信息

Devos R, Contreras R, van Emmelo J, Fiers W

出版信息

J Mol Biol. 1979 Mar 15;128(4):621-32. doi: 10.1016/0022-2836(79)90296-1.

DOI:10.1016/0022-2836(79)90296-1

PMID:374746

Abstract

摘要

相似文献

Identification of the translocatable element IS1 in a molecular chimera constructed with plasmid pBR322 DNA into which a bacteriophage MS2 DNA copy was inserted by the poly(dA).poly(dT) linker method.在用聚（dA）·聚（dT）连接法将噬菌体MS2 DNA拷贝插入质粒pBR322 DNA构建的分子嵌合体中鉴定可转座元件IS1。

J Mol Biol. 1979 Mar 15;128(4):621-32. doi: 10.1016/0022-2836(79)90296-1.

Construction and characterization of a plasmid containing a nearly full-size DNA copy of bacteriophage MS2 RNA.

J Mol Biol. 1979 Mar 15;128(4):595-619. doi: 10.1016/0022-2836(79)90295-x.

[Termination of DNA transcription by a poly(dA).poly(dT) fragment inserted into pBR325 plasmid].[通过插入pBR325质粒的聚（dA）·聚（dT）片段终止DNA转录]

Mol Biol (Mosk). 1984 May-Jun;18(3):599-606.

Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).质粒插入的聚（dA）·聚（dT）上的核小体重建

EMBO J. 1982;1(2):173-9. doi: 10.1002/j.1460-2075.1982.tb01143.x.

A unique deletion in pBR322 DNA caused by insertion of poly(dA).poly(dT).

Plasmid. 1989 May;21(3):247-50. doi: 10.1016/0147-619x(89)90049-8.

Excision of DNA segments introduced into cloning vectors by the poly(dA-dT) joining method.通过聚（dA-dT）连接法引入克隆载体的DNA片段的切除。

Proc Natl Acad Sci U S A. 1978 Apr;75(4):1763-7. doi: 10.1073/pnas.75.4.1763.

Specific excision of the inserted DNA segment from hybrid plasmids constructed by the poly(dA). poly (dT) method.通过聚（dA）·聚（dT）方法构建的杂交质粒中插入的DNA片段的特异性切除。

Biochim Biophys Acta. 1976 Dec 13;454(3):587-91. doi: 10.1016/0005-2787(76)90286-0.

[Effect of poly(dA).poly(dT) sequence inserted into EcoR1 site of pBr322 plasmid on the expression of tet and bla genes].

Mol Biol (Mosk). 1989 Jan-Feb;23(1):101-6.

Circular dichroism study of the interaction between T4 gene 32 protein and polynucleotides.

Biochemistry. 1978 Mar 7;17(5):887-93. doi: 10.1021/bi00598a022.

Nucleosomes will not form on double-stranded RNa or over poly(dA).poly(dT) tracts in recombinant DNA.核小体不会在双链RNA上形成，也不会在重组DNA中的聚（dA）·聚（dT）区段上形成。

Nucleic Acids Res. 1981 Dec 21;9(24):6869-88. doi: 10.1093/nar/9.24.6869.

引用本文的文献

Functional expression of individual plasmid-coded RNA bacteriophage MS2 genes.单个质粒编码的 RNA 噬菌体 MS2 基因的功能表达。

EMBO J. 1982;1(2):205-9. doi: 10.1002/j.1460-2075.1982.tb01148.x.

Gamma delta-mediated deletions of chromosomal segments on F-prime plasmids.γδ介导的F-prime质粒上染色体片段的缺失。

Mol Gen Genet. 1983;190(1):42-50. doi: 10.1007/BF00330322.

A cloned immunoglobulin cDNA fragment enhances transposition of IS elements into recombinant plasmids.一个克隆的免疫球蛋白cDNA片段增强了IS元件向重组质粒的转座作用。

Nucleic Acids Res. 1982 Aug 11;10(15):4525-42. doi: 10.1093/nar/10.15.4525.

On the molecular mechanisms of transposition.关于转座的分子机制

Proc Natl Acad Sci U S A. 1981 Aug;78(8):4858-62. doi: 10.1073/pnas.78.8.4858.

Does the insertion element IS1 transpose preferentially into A+T-rich DNA segments?插入元件IS1是否优先转座到富含A+T的DNA片段中？

Mol Gen Genet. 1980;178(2):471-3. doi: 10.1007/BF00270502.

IS4 at its chromosomal site in E. coli K12.大肠杆菌K12染色体位点上的IS4。

Mol Gen Genet. 1980;178(2):285-91. doi: 10.1007/BF00270474.

Recombination in recA cells between direct repeats of insertion element IS1.recA细胞中插入元件IS1的同向重复序列之间的重组。

J Bacteriol. 1985 May;162(2):529-34. doi: 10.1128/jb.162.2.529-534.1985.

Transposable element IS1 intrinsically generates target duplications of variable length.转座元件IS1本质上会产生长度可变的靶序列重复。

Proc Natl Acad Sci U S A. 1985 Feb;82(3):839-43. doi: 10.1073/pnas.82.3.839.

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