School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China; School of Life Sciences, Shanghai University, Shanghai 200444, China.
School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123143. doi: 10.1016/j.saa.2023.123143. Epub 2023 Jul 13.
Melamine is one of the common limited contaminations in dairy products. The traditional detection method has a long period and complicated pretreatment process. The rapid detection method is the better method to solve the screening of limited contaminations. In this paper, taking melamine as the research object, the surface enhanced Raman spectrum of melamine in liquid milk were collected by portable Raman spectrometer, and melamine was qualitatively identified and semi-quantitatively analyzed by Raman characteristic peak and Raman intensity, and a simple and efficient rapid screening method for limited contaminations was developed. The limit of detection is 0.25 mg/kg. The probability of detection is 100% at 2.5 mg/kg, which is the same between the two laboratories, indicating that the semi-quantitative method has good repeatability. The method of melamine proposed in this study can meet the rapid screening requirements of limited contaminations at the maximum residue limit, and has a good application prospect.
三聚氰胺是乳制品中常见的限量污染物之一。传统的检测方法周期长，预处理过程复杂。快速检测方法是解决限量污染物筛选的较好方法。本文以三聚氰胺为研究对象，采用便携式拉曼光谱仪采集了液态奶中三聚氰胺的表面增强拉曼光谱，通过特征峰和拉曼强度对三聚氰胺进行了定性鉴定和半定量分析，建立了一种简单高效的快速限量污染物筛选方法。该方法的检出限为 0.25mg/kg，在 2.5mg/kg 时的检出概率为 100%，两个实验室的结果一致，表明半定量方法具有良好的重复性。本研究提出的三聚氰胺检测方法可以满足最大残留限量限量污染物的快速筛选要求，具有良好的应用前景。
