Barraco R A, Phillis J W, Campbell W R, Marcantonio D R, Salah R S
Neuropharmacology. 1986 Jul;25(7):675-80. doi: 10.1016/0028-3908(86)90081-x.
Rats were implanted with chronic indwelling cannulae into the lateral cerebral ventricle. After recovery from surgery, acute experiments on blood pressure were conducted under methoxyflurane/nitrous oxide anesthesia. Rats were injected intracerebroventricularly with two adenosine analogs, 5'-N-ethylcarboxaminidoadenosine (NECA) and (-)-N-(1-methyl-2-phenylethyl)adenosine(L-phenylisopropyladenosine) (L-PIA), and the effects on blood pressure and heart rate recorded. Both analogs produced dose-related reductions in blood pressure and heart rate with L-PIA producing a more potent depression of heart rate than NECA. These effects on blood pressure and heart rate were antagonized by parenteral injections of caffeine. In separate experiments, the responses of blood pressure and heart rate to microinjection of NECA into the brainstem of rats anaesthetized with methoxyflurane/nitrous oxide were also examined. Microinjection of 2.7 nmol/kg into the fourth ventricle in the region of the area postrema produced a profound and long-lasting depression of blood pressure and heart rate. These results show that central injections of analogs of adenosine can influence the areas of the central nervous system involved in the control of cardiovascular function.
将慢性留置套管植入大鼠侧脑室。术后恢复后，在甲氧氟烷/一氧化二氮麻醉下进行急性血压实验。向大鼠脑室内注射两种腺苷类似物，即5'-N-乙基甲酰胺基腺苷（NECA）和（-）-N-（1-甲基-2-苯乙基）腺苷（L-苯异丙基腺苷）（L-PIA），并记录对血压和心率的影响。两种类似物均使血压和心率呈剂量依赖性降低，其中L-PIA对心率的抑制作用比NECA更强。静脉注射咖啡因可拮抗这些对血压和心率的影响。在单独的实验中，还研究了在甲氧氟烷/一氧化二氮麻醉的大鼠脑干中微量注射NECA时血压和心率的反应。在最后区区域向第四脑室微量注射2.7 nmol/kg可使血压和心率产生深刻而持久的降低。这些结果表明，向中枢注射腺苷类似物可影响参与心血管功能控制的中枢神经系统区域。
