School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
Chem Biodivers. 2023 Aug;20(8):e202300793. doi: 10.1002/cbdv.202300793. Epub 2023 Aug 2.
The utilization of rhizomes from the genus Atractylodes has been challenging due to their closely related origins. In this study, we developed an analytical strategy to differentiate Atractylodes lancea (A. lancea), Atractylodes chinensis (A. chinensis), Atractylodes japonica (A. japonica), and Atractylodes macrocephala (A. macrocephala), and compared their volatile compositions. Gas chromatography-mass spectrometry (GC/MS) was used to analyze the volatile profiles of essential oils extracted from 59 batches of samples. Chemometric methods enabled a better understanding of the differences in volatile oils between the four species and identified significant components affecting their classification and quality. A total of 50 volatile components were identified from the essential oils by GC/MS. Unsupervised and supervised chemometric analyses accurately distinguished A. lancea, A. chinensis, A. japonica, and A. macrocephala. Furthermore, five characteristic chemical markers, namely hinesol, β-eudesmol, atractylon, atractylodin and atractylenolide I, were obtained, and their respective percentage contents in individual species and samples were determined. This study provides a valuable reference for the quality evaluation of medicinal plants with essential oils and holds significance for species differentiation and the rational clinical application of Atractylodes herbs.
由于根茎来源密切相关，利用白术属根茎一直具有挑战性。在这项研究中，我们开发了一种分析策略来区分白术（A. lancea）、白术（A. chinensis）、白术（A. japonica）和白术（A. macrocephala），并比较了它们的挥发性成分。气相色谱-质谱联用（GC/MS）用于分析从 59 批样品中提取的精油的挥发性图谱。化学计量学方法有助于更好地理解四种物种之间精油的差异，并确定了影响它们分类和质量的重要成分。通过 GC/MS 从精油中鉴定出 50 种挥发性成分。无监督和有监督的化学计量学分析准确地区分了白术、白术、白术和白术。此外，还获得了 5 种特征性化学标志物，即灵香草醇、β-桉叶醇、苍术酮、苍术素和白术内酯 I，并确定了它们在各个物种和样品中的百分含量。这项研究为具有精油的药用植物的质量评价提供了有价值的参考，对物种分化和白术类草药的合理临床应用具有重要意义。
