Guo Lan-ping, Liu Jun-ying, Ji Li, Huang Lu-qi
Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2002 Nov;27(11):814-9.
To find the chemical diversity and characteristics of A. lancea on two levels--individuals and populations, and to discover the chemical essentials for forming geoherbs.
47 rhizomes of A. lancea were collected in 7 populations, and 6 naphtha components (1. elemol, 2. hinesol, 3. beta-eudesmol, 4. atractylone, 5. atractylodin, 6. atractylenolid I) in the rhizomes were determined by GC-MS combination. Principal Component Analysis and Cluster Analysis were carried out by SPSS.
Cluster Analysis of the 6 main components indicated that the chemical components of geoherbs were different from those of the non-geonerbs of A. lancea. Other analysis showed as follows: 1. The general oil of geoberbs were lower than that of non-geoherbs(P < 0.01), but components yielding more than 1% (% of the total oil) were more than non-geoherbs(P < 0.01); 2. Hinesol mixing beta-eudesmol was more in non-geoherbs, which atractylodin mixing atractylone was more in geoherbs(P < 0.001); 3. Principal Component Analysis implied that atractylone was the most important component to discriminate geoherbs and non-geoherbs of A. Lancea.
The naphtha composing characteristics of geoherbs was the special proportionment sale, viz. atractylone: hinesol: beta-eudesmol: atractylodin being(0.702.00):(0.040.35):(0.09~0.40):1.
从个体和种群两个层面探究茅苍术的化学多样性及特征，找出形成道地药材的化学要素。
采集7个种群的47份茅苍术根茎，采用气相色谱 - 质谱联用仪测定根茎中的6种挥发油成分（1. 榄香烯、2. 苍术醇、3. β-桉叶醇、4. 苍术酮、5. 苍术素、6. 苍术内酯Ⅰ）。运用SPSS软件进行主成分分析和聚类分析。
对6种主要成分进行聚类分析表明，茅苍术道地药材与非道地药材的化学成分存在差异。其他分析结果如下：1. 道地药材的总挥发油含量低于非道地药材（P < 0.01），但含量超过1%（占总挥发油的百分比）的成分比非道地药材多（P < 0.01）；2. 苍术醇与β-桉叶醇在非道地药材中含量较多，而苍术素与苍术酮在道地药材中含量较多（P < 0.001）；3. 主成分分析表明，苍术酮是区分茅苍术道地药材与非道地药材的最重要成分。
茅苍术道地药材挥发油的组成特征为特殊的比例关系，即苍术酮∶苍术醇∶β-桉叶醇∶苍术素为(0.702.00)∶(0.040.35)∶(0.09~0.40)∶1。
