白术属植物的随机扩增多态性DNA分析及精油成分变异

Random amplified polymorphic DNA analysis and variation of essential oil components of Atractylodes plants.

作者信息

Kohjyouma M, Nakajima S, Namera A, Shimizu R, Mizukami H, Kohda H

机构信息

The Experimental Station of Medicinal Plants, Hiroshima University School of Medicine, Minami-ku, Japan.

出版信息

Biol Pharm Bull. 1997 May;20(5):502-6. doi: 10.1248/bpb.20.502.

DOI:10.1248/bpb.20.502

PMID:9178929

Abstract

Total DNAs were prepared from the leaves of Atractylodes lancea DE CANDOLLE, A. chinensis KOIDZUMI, A. lancea var. simplicifolia KITAMURA, A. japonica KOIDZUMI ex KITAMURA and A. ovata DE CANDOLLE. The DNAs were subjected to random amplified polymorphic DNA (RAPD) analysis. Some primers showed the definitive polymorphic DNA patterns in A. lancea, A. japonica and A. ovata. The RAPD of A. lancea var. simplicifolia and one of A. chinensis gave similar patterns to those of A. lancea, but one of the other A. chinensis gave a similar pattern to A. japonica. Furthermore, quantitative analysis of atractylon, hinesol, beta-eudesmol and atractylodin in the rhizomes was done using gas chromatography. Though atractylon was detected not only in A. japonica and A. ovata but also in some of A. lancea, their RAPD profiles revealed the presence of intraspecific variation with A. lancea.

摘要

从茅苍术、北苍术、单叶苍术、关苍术和白术的叶片中提取总DNA。对这些DNA进行随机扩增多态性DNA（RAPD）分析。一些引物在茅苍术、关苍术和白术中显示出明确的多态性DNA图谱。单叶苍术和一种北苍术的RAPD图谱与茅苍术相似，但另一种北苍术的图谱与关苍术相似。此外，使用气相色谱法对根茎中的苍术酮、广藿香醇、β-桉叶醇和苍术素进行了定量分析。虽然不仅在关苍术和白术中检测到了苍术酮，在一些茅苍术中也检测到了，但它们的RAPD图谱显示茅苍术存在种内变异。