Bortz B M, Kenny G E, Pauley G B, Garcia-Ortigoza E, Anderson D P
Dev Comp Immunol. 1984 Fall;8(4):813-22. doi: 10.1016/0145-305x(84)90064-8.
The enzyme-linked immunosorbent assay (ELISA) procedure was modified and adapted for detection of circulating antibodies in rainbow trout (Salmo gairdneri) against metacercariae of the digenean trematode Diplostomum spathaceum, the causative agent for diplostomiasis. Rainbow trout (Salmo gairdneri) were injected with sonicated metacercariae representing 10, 40, and 100 metacercariae per fish. Three weeks after immunization the average titers for trout injected with 10, 40, and 100 metacercariae were 874, 841, and 525, and by six weeks the titers had fallen to 299, 349, and 203, respectively. Nine weeks after initial immunization, two remaining fish initially immunized with 100 metacercariae per fish were injected with a booster of 50 sonicated metacercariae per fish. Four weeks later the average titer was 1204. Serum samples from naturally infected wild fish tested for the presence of circulating antibodies against Diplostomum spathaceum showed 25 of 27 with positive titers.
酶联免疫吸附测定（ELISA）程序经过修改，适用于检测虹鳟鱼（Salmo gairdneri）体内针对复殖吸虫双穴吸虫（Diplostomum spathaceum）囊蚴的循环抗体，双穴吸虫是双穴吸虫病的病原体。给虹鳟鱼（Salmo gairdneri）注射经超声处理的囊蚴，每尾鱼分别注射相当于10、40和100个囊蚴的量。免疫三周后，注射10、40和100个囊蚴的虹鳟鱼平均滴度分别为874、841和525，到六周时，滴度分别降至299、349和203。初次免疫九周后，对最初每尾鱼注射100个囊蚴的剩余两条鱼，每尾再注射50个经超声处理的囊蚴作为加强免疫。四周后平均滴度为1204。对自然感染的野生鱼血清样本检测针对双穴吸虫囊蚴的循环抗体，结果显示27份样本中有25份滴度呈阳性。
