Klaysubun Chollachai, Srisuk Nantana, Duangmal Kannika
Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005665.
A polyphasic approach was used to describe strain RB6PN25, an actinobacterium isolated from peat swamp forest soil in Rayong Province, Thailand. The strain was a Gram-stain-positive and filamentous bacterium that contained -diaminopimelic acid, mannose and ribose in whole-cell hydrolysates. MK-9(H) was the major menaquinone. The major fatty acids were -C, -C and -C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified glycophospholipids, two unidentified aminolipids and an unidentified phospholipid. The 16S rRNA gene sequences analysis indicated that it was most closely related to DSM 42083 (97.6 %) and TBRC 1999 (97.4 %). Strain RB6PN25 exhibited low average nucleotide identity and digital DNA-DNA hybridization values with DSM 42083 (78.6 %, 23.2 %) and TBRC 1999 (76.0 %, 22.6 %). The DNA G+C content of strain RB6PN25 was 69.9%. The results of phenotypic, chemotaxonomic, genotypic and phylogenetic analyses reveal that strain RB6PN25 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RB6PN25 (=TBRC 14819=NBRC 115204).
采用多相分类方法对从泰国罗勇府泥炭沼泽森林土壤中分离出的放线菌菌株RB6PN25进行描述。该菌株为革兰氏阳性丝状细菌，全细胞水解产物中含有二氨基庚二酸、甘露糖和核糖。MK-9(H)是主要的甲基萘醌。主要脂肪酸为-C、-C和-C。极性脂质谱由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、两种未鉴定的糖磷脂、两种未鉴定的氨基脂质和一种未鉴定的磷脂组成。16S rRNA基因序列分析表明，它与DSM 42083（97.6%）和TBRC 1999（97.4%）关系最为密切。菌株RB6PN25与DSM 42083（78.6%，23.2%）和TBRC 1999（76.0%，22.6%）的平均核苷酸同一性和数字DNA-DNA杂交值较低。菌株RB6PN25的DNA G+C含量为69.9%。表型、化学分类、基因型和系统发育分析结果表明，菌株RB6PN25代表属的一个新物种，为此提出新物种名为sp. nov.。模式菌株为RB6PN25（=TBRC 14819=NBRC 115204）。
