A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia.
Inflammation. 2023 Dec;46(6):2306-2319. doi: 10.1007/s10753-023-01879-2. Epub 2023 Jul 25.
In recent years, there has been increasing interest in studying the anti-inflammatory activity of polyunsaturated fatty acid ethanolamides (N-acylethanolamines, NAE), which are highly active lipid mediators. The results of this study demonstrate that a dietary supplement (DS) of fatty acid-derived NAEs reduces LPS-induced inflammation. The processes of cell proliferation, as well as the dynamics of Iba-1-, CD68-, and CD163-positive macrophage activity within the thymus and spleen were studied. The production of pro-inflammatory cytokines (TNF, IL1β, IL6, and INFγ), ROS, NO, and nitrites was evaluated in the blood serum, thymus, and LPS-stimulated RAW264.7 mouse macrophages. In vitro and in vivo experiments have shown that DS (1) prevents LPS-induced changes in the morphological structure of the thymus and spleen; (2) levels out changes in cell proliferation; (3) inhibits the activity of Iba-1 and CD68-positive cells; (4) reduces the production of pro-inflammatory cytokines (TNF, IL1β, IL6, and INFγ), ROS, and CD68; and (5) enhances the activity of CD-163-positive cells. In general, the results of this study demonstrate the complex effect of DS on inflammatory processes in the central and peripheral immune systems.
近年来，人们对研究多不饱和脂肪酸乙醇酰胺（N-酰基乙醇胺，NAE）的抗炎活性越来越感兴趣，NAE 是一种高度活跃的脂质介质。本研究结果表明，脂肪酸衍生的 NAE 膳食补充剂可减轻 LPS 诱导的炎症。研究了细胞增殖过程以及胸腺和脾脏中 Iba-1、CD68 和 CD163 阳性巨噬细胞活性的动力学。在血清、胸腺和 LPS 刺激的 RAW264.7 小鼠巨噬细胞中评估了促炎细胞因子（TNF、IL1β、IL6 和 INFγ）、ROS、NO 和亚硝酸盐的产生。体内外实验表明，DS（1）可预防 LPS 诱导的胸腺和脾脏形态结构变化；（2）使细胞增殖变化趋于正常；（3）抑制 Iba-1 和 CD68 阳性细胞的活性；（4）减少促炎细胞因子（TNF、IL1β、IL6 和 INFγ）、ROS 和 CD68 的产生；（5）增强 CD-163 阳性细胞的活性。总的来说，本研究的结果表明 DS 对中枢和外周免疫系统炎症过程的复杂影响。
