Dorf Justyna, Pryczynicz Anna, Matowicka-Karna Joanna, Zaręba Konrad, Żukowski Piotr, Zalewska Anna, Maciejczyk Mateusz
Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland.
Department of General Pathomorphology, Medical University of Bialystok, Bialystok, Poland.
Front Oncol. 2023 Jul 12;13:1213802. doi: 10.3389/fonc.2023.1213802. eCollection 2023.
Nitrosative stress leads to protein glycoxidation, but both processes may be strongly related to the cancer development. Therefore, the aim of this study was to assess the nitrosative stress and protein glycoxidation products in patients with gastric cancer in comparison with healthy controls. We are also the first to evaluate the diagnostic utility of nitrosative stress and protein glycoxidation markers in gastric cancer patients in respect to histopathological classifications (TNM, Lauren's and Goseki's classification) and histopathological parameters such as histological type, histological differentiation grade, presence of vascular or neural invasion, desmoplasia and infection.
The study included 50 patients with gastric cancer and 50 healthy controls matched for sex and age. Nitrosative stress parameters and protein glycoxidation products were measured colorimetrically/fluorometrically in plasma or serum samples. Student's t-test or Mann-Whitney U-test were used for statistical analysis.
NO, S-nitrosothiols, nitrotyrosine, kynurenine, N-formylkynurenine, dityrosine, AGE and Amadori products were significantly increased whereas tryptophan fluorescence was decreased in patients with gastric cancer compared to the healthy control. Nitrosative stress and glycoxidation products may be useful in diagnosis of gastric cancer because they differentiate patients with gastric cancer from healthy individuals with high sensitivity and specificity. Some of the determined parameters are characterised by high AUC value in differentiation of GC patients according to the histopathological parameters.
Gastric cancer is associated with enhanced circulating nitrosative stress and protein glycation. Although further research on a tissue model is needed, plasma/serum biomarkers may be dependent on tumour size, histological type, tumour invasion depth, presence of lymph node and distant metastasis, vascular and neural invasion and Helicobacter pylori infection. Thus, circulating biomarkers of nitrosative stress/protein glycoxidation may have potential diagnostic significance in gastric cancer patients.
亚硝化应激会导致蛋白质糖氧化，而这两个过程都可能与癌症发展密切相关。因此，本研究的目的是评估胃癌患者与健康对照者的亚硝化应激及蛋白质糖氧化产物。我们也是首个评估亚硝化应激和蛋白质糖氧化标志物在胃癌患者中对于组织病理学分类（TNM、劳伦氏和御关氏分类）以及组织病理学参数（如组织学类型、组织学分化程度、血管或神经侵犯情况、促纤维增生反应和感染）的诊断效用。
本研究纳入了50例胃癌患者和50例年龄及性别匹配的健康对照者。采用比色法/荧光法测定血浆或血清样本中的亚硝化应激参数和蛋白质糖氧化产物。采用学生t检验或曼-惠特尼U检验进行统计分析。
与健康对照相比，胃癌患者的一氧化氮、S-亚硝基硫醇、硝基酪氨酸、犬尿氨酸、N-甲酰犬尿氨酸、二酪氨酸、晚期糖基化终末产物和阿马多里产物显著增加，而色氨酸荧光降低。亚硝化应激和糖氧化产物可能有助于胃癌的诊断，因为它们能以高灵敏度和特异性区分胃癌患者与健康个体。根据组织病理学参数，一些测定参数在区分胃癌患者方面具有较高的曲线下面积值。
胃癌与循环亚硝化应激增强和蛋白质糖基化有关。尽管需要对组织模型进行进一步研究，但血浆/血清生物标志物可能取决于肿瘤大小、组织学类型、肿瘤浸润深度、淋巴结和远处转移情况、血管和神经侵犯以及幽门螺杆菌感染。因此，循环亚硝化应激/蛋白质糖氧化生物标志物可能对胃癌患者具有潜在的诊断意义。
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