Tariq Mamoona, Khokhar Rabia, Javed Arslan, Usman Muhammad, Anjum Syed Muhammad Muneeb, Rasheed Huma, Bukhari Nadeem Irfan, Yan Chao, Nawaz Hafiz Awais
School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, China.
Punjab University College of Pharmacy (PUCP), University of the Punjab, Lahore 54000, Pakistan.
Gels. 2023 Jul 11;9(7):564. doi: 10.3390/gels9070564.
Gelatin-based hydrogels have shown good injectability and biocompatibility and have been broadly used for drug delivery and tissue regeneration. However, their low mechanical strengths and fast degradation rates must be modified for long-term implantation applications. With an aim to develop mechanically stable hydrogels, reactive anhydride-based oligomers were developed and used to fabricate gelatin-based crosslinked hydrogels in this study. A cascade of hydrophilic oligomers containing reactive anhydride groups was synthesized by free radical polymerization. These oligomers varied in degree of reactivity, comonomer composition, and showed low molecular weights (M < 5 kDa). The reactive oligomers were utilized to fabricate hydrogels that differed in their mechanical strengths and degradation profiles. These formulations exhibited good cytocompatibility with human adipose tissue-derived stem cells (hADCs). In conclusion, the reactive MA-containing oligomers were successfully synthesized and utilized for the development of oligomer-crosslinked hydrogels. Such oligomer-crosslinked gelatin-based hydrogels hold promise as drug or cell carriers in various biomedical applications.
基于明胶的水凝胶已显示出良好的可注射性和生物相容性，并已广泛用于药物递送和组织再生。然而，对于长期植入应用，必须对其低机械强度和快速降解率进行改进。为了开发机械稳定的水凝胶，本研究中开发了基于反应性酸酐的低聚物，并用于制备基于明胶的交联水凝胶。通过自由基聚合合成了一系列含有反应性酸酐基团的亲水性低聚物。这些低聚物的反应程度、共聚单体组成各不相同，且分子量较低（M < 5 kDa）。利用这些反应性低聚物制备了机械强度和降解特性不同的水凝胶。这些配方对人脂肪组织来源的干细胞（hADCs）表现出良好的细胞相容性。总之，成功合成了含反应性甲基丙烯酸酯的低聚物，并将其用于开发低聚物交联水凝胶。这种低聚物交联的基于明胶的水凝胶有望在各种生物医学应用中作为药物或细胞载体。
