Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia.
J Periodontol. 2021 Jun;92(6):875-885. doi: 10.1002/JPER.20-0205. Epub 2020 Sep 21.
Decontamination of biofilm-infected rough implant surfaces is challenging. Platelet rich blood products have been shown to have anti-microbial properties against periodontal pathogens. Our aim was to investigate the effect of a potential biological implant surface disinfectant, leukocyte- and platelet-rich fibrin (L-PRF), on a mature oral multispecies biofilm on a rough titanium surface.
Sandblasted, large grit, acid-etched (SLA) titanium disks were inoculated with subgingival dental plaque and cultured anaerobically for 21 days. The L-PRF membranes were collected from 12 donors in three trials (four donors in each trial). The disks were rinsed with 0.9% NaCl and exposed to the cell-rich portion of the L-PRF membranes for 48 hours followed by scanning electron microscope (SEM) analysis immediately or after rinsing with 0.9% NaCl prior to fixation. The presence of platelet factor-4 in the rinse samples was analyzed by Western blotting. Remaining bacteria were quantified from SEM images of the implant surfaces and their numbers statistically compared.
The L-PRF-treated samples without rinsing displayed numerous cells with multiple pseudopodia in immediate contact with bacteria that appeared perforated and increased in size. The cells were identified as platelets based on morphological criteria and by positive reaction for platelet factor-4 by Western blotting. After post-treatment rinsing, the L-PRF-treated disks displayed a significant reduction in bacterial counts (in average 92% reduction).
Application of L-PRF significantly reduced bacterial counts on contaminated SLA titanium surface, most likely through anti-microbial action by platelets.
生物膜感染的粗糙种植体表面的去污是具有挑战性的。富含血小板的血液制品已被证明具有抗牙周病原体的抗菌特性。我们的目的是研究潜在的生物种植体表面消毒剂富白细胞和血小板纤维蛋白（L-PRF）对粗糙钛表面成熟口腔多物种生物膜的影响。
喷砂酸蚀（SLA）钛盘用龈下牙菌斑接种，并在厌氧条件下培养 21 天。从 12 名供体的三次试验中收集 L-PRF 膜（每次试验中有 4 名供体）。将圆盘用 0.9%NaCl 冲洗，并将富含细胞的 L-PRF 膜部分暴露于其中 48 小时，然后立即用扫描电子显微镜（SEM）分析，或在固定前用 0.9%NaCl 冲洗。通过 Western blot 分析冲洗样品中血小板因子-4 的存在。通过 SEM 图像定量分析种植体表面残留的细菌，并对其数量进行统计学比较。
未经冲洗的 L-PRF 处理样品显示出许多带有多个伪足的细胞，这些细胞与看起来穿孔且体积增大的细菌直接接触。根据形态学标准和 Western blot 血小板因子-4 的阳性反应，这些细胞被鉴定为血小板。经过后处理冲洗后，L-PRF 处理的圆盘显示细菌计数显著减少（平均减少 92%）。
L-PRF 的应用显著降低了污染 SLA 钛表面的细菌计数，这很可能是通过血小板的抗菌作用。
