Öncü E, Bayram B, Kantarci A, Gülsever S, Alaaddinoğlu E-E
Karaciğan Mah.Ankara, cad, Necmettin Erbakan Üniversitesi, Diş Hekimliği Fakültesi Karatay, Konya- Turkey,
Med Oral Patol Oral Cir Bucal. 2016 Sep 1;21(5):e601-7. doi: 10.4317/medoral.21026.
Leukocyte-platelet rich fibrin (L-PRF) is a second generation platelet concentrate clinically used to accelerate tissue healing and bone regeneration. Achieving reduced implant osseointegration time could provide immediate or early loading of implants. The aim of this study was to evaluate the L-PRF-induced osseointegration and bone-implant contact (BIC) in an experimental animal model.
Twelve 4-month-old New Zealand white rabbits were used. Following general anesthesia, 3-5 mL of blood was obtained from the central artery in rabbit ear and L-PRF was prepared. Two implant cavities (5 mm long and 3 mm in diameter) were created in each tibia with a total of four cavities in each animal. Two of these cavities were selected and covered with PRF (test group). The remaining L-PRF was used to soak the implants placed into the L-PRF covered sockets. Other cavities were left as controls. In total, 48 implants were placed. Animals were sacrificed after two, three, or four weeks. Histological samples were obtained and peri-implant tissues were histomorphometrically evaluated for bone-to-implant contact and new bone formation.
Histomorphometric analyses of the defects revealed that the L-PRF was detectable up to the second week. Application of L-PRF increased the rate and amount of new bone formation in the experimental group compared to the control group. Bone-to-implant contact was enhanced when the surface was pre-wetted with L-PRF (p<0.01).
The results of this study demonstrated that L-PRF application may increases amount and rate of new bone formation during the early healing period and provides a faster osseointegration around implants.
富白细胞血小板纤维蛋白（L-PRF）是第二代血小板浓缩物，临床上用于加速组织愈合和骨再生。缩短种植体骨整合时间可实现种植体的即刻或早期加载。本研究的目的是在实验动物模型中评估L-PRF诱导的骨整合和骨-种植体接触（BIC）。
使用12只4月龄的新西兰白兔。全身麻醉后，从兔耳中央动脉采集3-5 mL血液并制备L-PRF。每只胫骨制备两个种植窝（长5 mm，直径3 mm），每只动物共四个种植窝。选择其中两个种植窝并用PRF覆盖（试验组）。将剩余的L-PRF用于浸泡植入L-PRF覆盖种植窝的种植体。其他种植窝作为对照。共植入48枚种植体。在2、3或4周后处死动物。获取组织学样本，对种植体周围组织进行组织形态计量学评估，以分析骨-种植体接触和新骨形成情况。
对缺损的组织形态计量学分析显示，L-PRF在第二周仍可检测到。与对照组相比，试验组应用L-PRF可提高新骨形成的速率和数量。当种植体表面用L-PRF预湿润时，骨-种植体接触增强（p<0.01）。
本研究结果表明，应用L-PRF可能会增加早期愈合期间新骨形成的数量和速率，并使种植体周围的骨整合更快。
