Land G A, Harrison B A, Hulme K L, Cooper B H, Byrd J C
J Clin Microbiol. 1979 Sep;10(3):357-64. doi: 10.1128/jcm.10.3.357-364.1979.
The new API 20C yeast identification system together with appropriate microscopic morphology determinations achieved a 97% correlation with a rapid conventional method. Whereas a group composed of Candida, Torulopsis, Saccharomyces, and Rhodotorula was identified with ease (98% overall correlation), a second group, containing Cryptococcus, Trichosporon, and Geotrichum species, appeared to give the system the most difficulty (90% correlation). Within this group particular difficulty was encountered in identifying varieties of Cryptococcus albidus, C. terreus, C. laurentii, Trichosporon beigelli, and Geotrichum spp. as to species. The API 20C system should be incubated the full 72 h prescribed by the manufacturer. However, when used in conjunction with appropriate morphological tests, presumptive identifications of some Candida and Torulopsis species may be made at 24 to 48 h. To facilitate identifications of the more difficult group of yeasts, ancillary tests for determining nitrate reductase, urease, and phenol oxidase activities should be considered as additions to the strip. Incorporating the phenol oxidase test would be especially important for identification of Cryptococcus neoformans, a yeast which should be identified as quickly and as accurately as possible. The API 20C system with computer assistance has proved to be an easy-to-inoculate, versatile, and fairly rapid method of yeast identification, giving results comparable to those obtained by conventional methodologies.
新型API 20C酵母鉴定系统与适当的显微镜形态学判定相结合,与一种快速传统方法的符合率达到了97%。由假丝酵母属、球拟酵母属、酿酒酵母属和红酵母属组成的一组酵母很容易被鉴定出来(总体符合率为98%),而包含隐球菌属、丝孢酵母属和地霉属菌种的第二组酵母似乎给该系统带来了最大困难(符合率为90%)。在这一组中,在将白色隐球菌、土生隐球菌、罗伦隐球菌、白吉利丝孢酵母和地霉属的不同变种鉴定到种时遇到了特别的困难。API 20C系统应按照制造商规定的时间进行完整的72小时培养。然而,当与适当的形态学检测结合使用时,一些假丝酵母属和球拟酵母属菌种在24至48小时即可做出初步鉴定。为便于鉴定较难的酵母组,应考虑将用于测定硝酸还原酶、脲酶和酚氧化酶活性的辅助检测作为试条检测的补充。加入酚氧化酶检测对于新型隐球菌的鉴定尤为重要,新型隐球菌是一种应尽快且准确鉴定的酵母。事实证明,带有计算机辅助的API 20C系统是一种易于接种、用途广泛且相当快速的酵母鉴定方法,其结果与传统方法相当。