Department of Trauma Surgery, Orthopaedics and Plastic Surgery, University Medical Center Goettingen, Georg-August-University, 37075 Goettingen, Germany.
Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, 28359 Bremen, Germany.
Int J Mol Sci. 2023 Jul 18;24(14):11608. doi: 10.3390/ijms241411608.
Most implants used in trauma surgery are made of steel and remain inside the body only temporarily. The strong tissue interaction of such implants sometimes creates problems with their explantation. Modified implant surfaces, which decrease tissue attachment, might allow an easier removal and therefore a better outcome. Such a modification must retain the implant function, and needs to be biocompatible and cost-effective. Here, we used a novel VUV-light (Vacuum-Ultraviolett)-based coating technology (LightPLAS) to generate coated stainless-steel plates. The tested LightPLAS coating only had an average thickness of around 335 nm, making it unlikely to interfere with implant function. The coated plates showed good biocompatibility according to ISO 10993-5 and ISO 10993-12, and reduced cell adhesion after four different time points in a 2D cell culture system with osteoblast-like MG-63 cells. Furthermore, we could show decreased cell adhesion in our 3D cell culture system, which mimics the fluid flow above the implant materials as commonly present in the in vivo environment. This new method of surface coating could offer extended options to design implant surfaces for trauma surgery to reduce cell adhesion and implant ingrowth. This may allow for a faster removal time, resulting in shorter overall operation times, thereby reducing costs and complication rates and increasing patient wellbeing.
大多数用于创伤外科的植入物由钢制成，并且仅暂时留在体内。这种植入物与组织的强烈相互作用有时会导致其取出困难。改良的植入物表面可以减少组织附着，从而更容易取出，因此效果更好。这种改良必须保留植入物的功能，并且需要具有生物相容性和成本效益。在这里，我们使用了一种新型的 VUV 光（真空紫外线）基涂层技术（LightPLAS）来生成涂层不锈钢板。测试的 LightPLAS 涂层的平均厚度仅约为 335nm，不太可能干扰植入物的功能。根据 ISO 10993-5 和 ISO 10993-12，涂层板表现出良好的生物相容性，并且在使用成骨样 MG-63 细胞的 2D 细胞培养系统中，经过四个不同时间点后，细胞粘附减少。此外，我们可以在 3D 细胞培养系统中显示出减少的细胞粘附，该系统模拟了植入物材料上方的流体流动，这在体内环境中很常见。这种新的表面涂层方法可为创伤外科设计植入物表面提供更多选择，以减少细胞粘附和植入物内生长。这可能允许更快的取出时间，从而缩短总手术时间，从而降低成本和并发症发生率，并提高患者的幸福感。
