Pérez-Ochoa Mónica L, Vera-Guzmán Araceli M, Mondragón-Chaparro Demetria M, Sandoval-Torres Sadoth, Carrillo-Rodríguez José C, Mayek-Pérez Netzahualcoyotl, Chávez-Servia José L
CIIDIR-Oaxaca, Instituto Politécnico Nacional, Gustavo A. Madero, Ciudad de Mexico 07320, Mexico.
Instituto Tecnológico del Valles de Oaxaca, Santa Cruz Xoxocotlán, Oaxaca 71233, Mexico.
Plants (Basel). 2023 Sep 6;12(18):3192. doi: 10.3390/plants12183192.
Medicinal plants grown in natural settings are exposed to different adverse environmental conditions that determine their growth and development as well as the composition and concentration of secondary metabolites in their organs. The objective of this study was to evaluate the effects of environmental conditions associated with localities and annual growth cycles on the contents of phenolic compounds and flavonoids, antioxidant activity and potentially bioactive phenolic acids in the roots of , a medicinal species from temperate Mexico. The samples for composition analysis were collected using a bifactorial design: Factor A consisted of the localities (Morelos and La Unión de San Martin Huamelulpam, Mexico) and Factor B was represented by the annual growth cycle (2020 and 2021). In each sample, the contents of polyphenols and equivalent flavonoids of quercetin and catechin and antioxidant activity were evaluated using spectrophotometry. Subsequently, chlorogenic, caffeic and rosmarinic acids were identified and quantified using high-performance liquid chromatography with diode-array detection (HPLC-DAD). The annual growth conditions and, to a lesser extent, the locality of origin of the samples significantly influenced the contents of phenolic compounds and antioxidant activity. The environmental conditions that occurred in 2021 favored an increase in the contents of phenolic compounds compared to those in 2020, and the same pattern was observed for chlorogenic acid; however, for caffeic and rosmarinic acids, the opposite pattern was observed. The content of phenolic acids in the roots of follows different and independent patterns between cycles based on the interaction between the locality of origin and annual growth cycle. This study quantifies the magnitude of the total environmental effect on the phenolic compound concentrations in roots, which was measured via sampling during two annual growth cycles, where the sampling locations factor had little influence. The bioactive compounds identified in roots have the potential for use as alternative medicines, as mentioned by different families from Oaxaca, Mexico.
生长在自然环境中的药用植物会面临不同的不利环境条件，这些条件决定了它们的生长发育以及其器官中次生代谢产物的组成和浓度。本研究的目的是评估与产地和年度生长周期相关的环境条件对一种来自墨西哥温带的药用植物根部酚类化合物和黄酮类化合物的含量、抗氧化活性以及潜在生物活性酚酸的影响。用于成分分析的样品采用双因素设计采集：因素A为产地（墨西哥的莫雷洛斯和圣马丁瓦梅卢尔帕姆联合镇），因素B以年度生长周期（2020年和2021年）表示。在每个样品中，使用分光光度法评估多酚、槲皮素和儿茶素的当量黄酮含量以及抗氧化活性。随后，使用带二极管阵列检测的高效液相色谱法（HPLC-DAD）对绿原酸、咖啡酸和迷迭香酸进行鉴定和定量。年度生长条件以及在较小程度上样品的产地对酚类化合物的含量和抗氧化活性有显著影响。与2020年相比，2021年出现的环境条件有利于酚类化合物含量的增加，绿原酸也呈现相同模式；然而，对于咖啡酸和迷迭香酸，观察到相反的模式。基于产地和年度生长周期之间的相互作用，该药用植物根部酚酸的含量在不同周期遵循不同且独立的模式。本研究通过在两个年度生长周期内采样，量化了总环境对该药用植物根部酚类化合物浓度的影响程度，其中采样地点因素的影响较小。如墨西哥瓦哈卡不同家族所述，在该药用植物根部鉴定出的生物活性化合物有作为替代药物使用的潜力。
