González-Magallanes Berenice, Magaña-Guerrero Fátima Sofía, Bautista-de Lucio Victor Manuel, Hernández-Gómez Jimmy Giovanni, Salas-Lais Angel Gustavo, Hernández-Sánchez Humberto
Unidad de Investigación del Instituto de Oftalmología "Fundación de Asistencia Privada Conde de Valenciana I.A.P.", Chimalpopoca 14, Obrera, Mexico City 06800, Mexico.
Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Wilfrido Massieu Av. C.P., Mexico City 07738, Mexico.
Microorganisms. 2023 Jun 23;11(7):1644. doi: 10.3390/microorganisms11071644.
Probiotics play an important role against infectious pathogens, such as (), mainly through the production of antimicrobial compounds and their immunomodulatory effect. This protection can be detected both on the live probiotic microorganisms and in their inactive forms (paraprobiotics). Probiotics may affect different cells involved in immunity, such as macrophages. Macrophages are activated through contact with microorganisms or their products (lipopolysaccharides, endotoxins or cell walls). The aim of this work was the evaluation of the effect of two probiotic bacteria ( Nissle 1917 and subsp. HN019 on macrophage cell line J774A.1 when challenged with two pathogenic strains of . Macrophage activation was revealed through the detection of reactive oxygen (ROS) and nitrogen (RNS) species by flow cytometry. The effect varied depending on the kind of probiotic preparation (immunobiotic, paraprobiotic or postbiotic) and on the strain of (enterohemorrhagic or enteropathogenic). A clear immunomodulatory effect was observed in all cases. A higher production of ROS compared with RNS was also observed.
益生菌对传染性病原体，如（），起着重要作用，主要通过产生抗菌化合物及其免疫调节作用。这种保护作用在活的益生菌微生物及其无活性形式（副益生菌）中均能检测到。益生菌可能会影响参与免疫的不同细胞，如巨噬细胞。巨噬细胞通过与微生物或其产物（脂多糖、内毒素或细胞壁）接触而被激活。这项工作的目的是评估两种益生菌（Nissle 1917和亚种HN019）在受到两种致病菌株攻击时对巨噬细胞系J774A.1的影响。通过流式细胞术检测活性氧（ROS）和活性氮（RNS）来揭示巨噬细胞的激活情况。效果因益生菌制剂的种类（免疫益生菌、副益生菌或后生元）和菌株（肠出血性或肠致病性）而异。在所有情况下均观察到明显的免疫调节作用。与RNS相比，还观察到ROS的产生量更高。
